Overview

  • Product name
    Anti-E. coli antibody (Biotin)
    See all E. coli primary antibodies
  • Description
    Rabbit polyclonal to E. coli (Biotin)
  • Host species
    Rabbit
  • Conjugation
    Biotin
  • Specificity
    Many "O" and "K" antigenic serotypes of Escherichia coli. Will remove E. coli proteins from recombinant preparations. Tested specifically with the strains O18, O20, O44, O55, O111, O112, O125, O157, K12.
  • Tested applications
    Suitable for: ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Tissue/ cell preparation (Mixture of E. coli serotypes: K12, O111, O55, O125, O20, and O157).

  • General notes

    Covalently coupled with the N-Hydroxysuccinimide ester of biotin under mild conditions to give a high degree of substitution.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    Protein A chromatography.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.
  • Alternative names
    • Escherichia coli antibody

References

This product has been referenced in:
  • Migliore F  et al. Phagocytosis and Epithelial Cell Invasion by Crohn's Disease-Associated Adherent-Invasive Escherichia coli Are Inhibited by the Anti-inflammatory Drug 6-Mercaptopurine. Front Microbiol 9:964 (2018). Read more (PubMed: 29867868) »
  • Kokkinis G  et al. A microfluidic, dual-purpose sensor for in vitro detection of Enterobacteriaceae and biotinylated antibodies. Lab Chip 16:1261-71 (2016). Read more (PubMed: 26939996) »
See all 14 Publications for this product

Customer reviews and Q&As

1-10 of 13 Abreviews or Q&A

Question
Answer

The biotinylation of ab20640 was accomplished by covalently coupling the primary antibody free amine groups with the N-Hydroxysuccinimide ester of biotin under mild conditions to give a high degree of substitution.

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Answer

Thank you very much for your reply.

I'm sending a free of charge vial of ab137967 on the order ***. This antibody is back-ordered until September 10, and I expect it to arrive shortly after that date.

Please note that as we have not tested this antibody in your assay, we can not guarantee it to work, though I am very hopeful it will work better than ab68451. Please keep me updated about the results, and let me know if there is anything else that we can do for you.

Have a nice day!

Read More

Answer

Thank you again for all of your patience while we've worked toadd the unconjugated rabbit antibody to our catalog.

This is antibody is now availableas Rabbit polyclonal to E. coli (ab137967)-

https://www.abcam.com/E-coli-antibody-ab137967.html

Please keep me updatedabout the results withthis antibody in your pulldown assay, and let me know if there is anything else that we can do for you.

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Question
Answer

Thank you for contacting Abcam earlier today.

I have talked to the lab about your enquiry regarding ab20640 and they have informed me that the antibody has been tested and is reactive with cell surface antigens on intact E. coli organisms. The immunogen is a mixture of whole cells from several E.coli serotypes, but some of the organisms in the immunogen may become disrupted during the immunization process. The antibody is a polyclonal antibody and will therefore recognize multiple epitopes on multiple proteins. Some of the epitopes recognized may be on E. coli proteins that are not cell surface proteins, but this has not been tested.

Please let me know if there is anything else I can help you with.

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Question
Answer

Thank you for contacting us. Unfortunately, the biotin/antibody ration is not determined for this antibody. As I mentioned, a common estimate for antibody biotinylation in general is is 3 - 6 biotin molecules per immunoglobulin. Please let us know if you need any other information.

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Answer

Thank you for contacting Abcam.
For ab25823, it should be fine to conjugate the antibody with biotin, using one of our EasyLink kits.
As for ab20640, it may react with BL21, but I cannot guarantee it, all we know is that it will recognize the strains O18, O20, O44, O55, O111, O112, O125, O157, K12.
Please let me know if there is anything else I can help you with.

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Question
Answer

ab20640 is an IgG, so the MW without biotin is also ~150 kDa. I hope this helps.  

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Answer

Thank you for your enquiry. Unfortunately, it is not known exactly how much biotin per molecule of antibody is conjugated, the molecular weight of ab20640 is therefore undetermined. For ab35654, which is an unconjugated immunogloblulin, the molecular weight is ~150 kDa. As shown on the product datasheet, the concentration of ab20640 is 4 mg/ml and the concentration of ab35654 is 0.5 mg/ml. I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your enquiry. I can confirm that ab20640 Anti-E. coli antibody (Biotin) will recognize the K12 strain of E. coli. This antibody has been tested in ELISA against E.coli serotypes O157:H7, O20, O55, O111, O125 and K12. It will therefore be suitable for detection of the K12 strain. I hope this will be helpful to you. Should you have any further questions, please do not hesitate to contact us.

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Question
Answer

Thank you for your enquiry. Unfortunately, it is not known exactly how much biotin per molecule of antibody is congjugated. I wish that I had more information and apologize for this inconveniance. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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