Biotin Anti-E. coli antibody (ab20640)
Key features and details
- Biotin Rabbit polyclonal to E. coli
- Suitable for: ELISA, ICC/IF
- Reacts with: Escherichia coli
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-E. coli antibody
See all E. coli primary antibodies -
Description
Biotin Rabbit polyclonal to E. coli -
Host species
Rabbit -
Conjugation
Biotin -
Specificity
Many "O" and "K" antigenic serotypes of Escherichia coli. Will remove E. coli proteins from recombinant preparations. Tested specifically with the strains O18, O20, O44, O55, O111, O112, O125, O157, K12. -
Tested applications
Suitable for: ELISA, ICC/IFmore details -
Species reactivity
Reacts with: Escherichia coli -
Immunogen
Tissue, cells or virus corresponding to Escherichia coli E. coli. Mixture of E. coli serotypes: K12, O111, O55, O125, O20, and O157
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General notes
Covalently coupled with the N-Hydroxysuccinimide ester of biotin under mild conditions to give a high degree of substitution.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Protein A chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab20640 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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ELISA
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Relevance
Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins. -
Alternative names
- Escherichia coli antibody
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (24)
ab20640 has been referenced in 24 publications.
- Rodoplu D et al. A simple magnetic-assisted microfluidic method for rapid detection and phenotypic characterization of ultralow concentrations of bacteria. Talanta 230:122291 (2021). PubMed: 33934763
- Tan JMJ et al. Listeria exploits IFITM3 to suppress antibacterial activity in phagocytes. Nat Commun 12:4999 (2021). PubMed: 34404769
- Nie Y et al. Ursolic acid reverses liver fibrosis by inhibiting NOX4/NLRP3 inflammasome pathways and bacterial dysbiosis. Gut Microbes 13:1972746 (2021). PubMed: 34530693
- Leccese G et al. Probiotic Lactobacillus and Bifidobacterium Strains Counteract Adherent-Invasive Escherichia coli (AIEC) Virulence and Hamper IL-23/Th17 Axis in Ulcerative Colitis, but Not in Crohn's Disease. Cells 9:N/A (2020). PubMed: 32752244
- Smith S et al. Anti-Escherichia coli Functionalized Silver-Doped Carbon Nanofibers for Capture of E. coli in Microfluidic Systems. Polymers (Basel) 12:N/A (2020). PubMed: 32414196