• Product name
    Anti-E. coli antibody (Biotin)
    See all E. coli primary antibodies
  • Description
    Rabbit polyclonal to E. coli (Biotin)
  • Host species
  • Conjugation
  • Specificity
    ab68451 reacts with E. coli in bacterial and infected tissue samples.
  • Tested applications
    Suitable for: ELISAmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    O and K antigenic serotypes (All types) of Escherichia coli.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 0.01M PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab68451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.


  • Relevance
    Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.
  • Alternative names
    • Escherichia coli antibody


This product has been referenced in:
  • Dua P  et al. Cell-SELEX Based Identification of an RNA Aptamer for Escherichia coli and Its Use in Various Detection Formats. Mol Cells 39:807-813 (2016). Functional Studies . Read more (PubMed: 27871171) »
  • Biswas B  et al. Uropathogenic Escherichia coli (UPEC) induced antimicrobial gene expression in the male reproductive tract of rat: evaluation of the potential of Defensin 21 to limit infection. Andrology 3:368-75 (2015). ICC/IF . Read more (PubMed: 25675950) »

See all 2 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Flow Cytometry
Escherichia coli Cell (Escherichia Coli trapping using magnetic beads der)
Escherichia Coli trapping using magnetic beads der
Cell harvesting/tissue preparation method: magentic streptavidin-coated beads labeled with ab68451 (biotin) to trap E. Coli from dilutions after LB culture
Sample buffer: Phosphate Buffer Saline, pH=7.4 [0.01 M with 27 mM KCl, 137 mM NaCl]
on magnetic streptavidin beads

Cedric Hurth

Verified customer

Submitted Apr 07 2017

Thank you for contacting us.

I have attached the list of antibodies we have along with details. Please check.

I am sorry I am unable to confirm the species cross reactivity of the these antibodies other than waht is given on the datas...

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Thank you for contacting us.

ab41181 is anti Strep agalactiae, it will weakly cross react with Strep. dysgalactiae.

ab41203 is anti Strep agalactiae, it will weakly cross react with Strep. dysgalactiae.

ab19980 is an anti Ent...

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Thank you so much for your patience while I have worked on this enquiry.

I've looked through the literature, data on our E.coli antibodies, and also discussed with my colleagues, and I think that the unconjugated version of ab20640 will be th...

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Thank you for your reply.

I will get in touch with the lab about the unconjugated E. coli antibodies that we have, and see if there is any data to suggest that one will work in your assay! I'll get back in touch with you as soon as possible. ...

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Thank you very much for your reply.

I apologize for the confusion regarding this situation. For the offer that I've mentioned, the "review" is our online Abreview system. So, if you'd like to submit an Abreview for ab68451 (we appre...

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Thank you very much for your reply and for sending the description of the assay in which you will be using the E. coli antibody.

We have not tested any of our E. coli antibodies in a similar assay, so there isn't one in particular that we kno...

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Thank you for your reply.

I am very sorry that we do not have free samples to send, but our testing program is the option that weprovide for trying an antibody in an untested application.

We generally arrange this program prior to p...

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Thank you very much for your reply.

At this time, we do not have the unconjugated format of ab68451 in our catalog, but if you are interested in trying it Iwill see if we can add this antibodyto ourcatalog. We also have two unconjugated goat ...

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Thank you for your call last week and for your patience while I have been in touch with the lab regarding your enquiry.
I have confirmed with the lab that the biotinyalted antibody is tested, and I've obtained the following ELISA protocol from the...

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