Overview

  • Product name

    Anti-E. coli antibody (Biotin)
    See all E. coli primary antibodies
  • Description

    Rabbit polyclonal to E. coli (Biotin)
  • Host species

    Rabbit
  • Conjugation

    Biotin
  • Specificity

    ab68451 reacts with E. coli in bacterial and infected tissue samples.
  • Tested applications

    Suitable for: ELISAmore details
  • Species reactivity

    Reacts with: Escherichia coli
  • Immunogen

    O and K antigenic serotypes (All types) of Escherichia coli.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab68451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.

Target

  • Relevance

    Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.
  • Alternative names

    • Escherichia coli antibody

References

This product has been referenced in:

  • Dua P  et al. Cell-SELEX Based Identification of an RNA Aptamer for Escherichia coli and Its Use in Various Detection Formats. Mol Cells 39:807-813 (2016). Functional Studies . Read more (PubMed: 27871171) »
  • Biswas B  et al. Uropathogenic Escherichia coli (UPEC) induced antimicrobial gene expression in the male reproductive tract of rat: evaluation of the potential of Defensin 21 to limit infection. Andrology 3:368-75 (2015). ICC/IF . Read more (PubMed: 25675950) »
See all 2 Publications for this product

Customer reviews and Q&As

11-12 of 12 Abreviews or Q&A

Answer

Thank you for your call last week and for your patience while I have been in touch with the lab regarding your enquiry.
I have confirmed with the lab that the biotinyalted antibody is tested, and I've obtained the following ELISA protocol from the lab:
a) Fill wells of microtiter plate with 100 ul of bacterial suspension that is just visibly “hazy” in PBS or equivalent buffer. Incubate overnight uncovered@ 37 C in order to dry down the suspensions in the wells. Leave some wells uncoated as controls.
b) Wash wells 3 times with PBS containing 0.3% tween-20 detergent (PBST), or equivalent.
c) block wells with detergent solution containing 1 mg/ml BSA (gelatin or powdered, non-fat milk may be substituted) for a minimum of 1 hour @ RT.
d) repeat step b
e) add 100 ul of dilutions (made with PBST) of known postive and negative sera (or antibody being tested) to the various wells and incubate for 30 min. @ RT.
f) repeat step b
g) add 100 ul of Streptavidin-HRP diluted optimumly in PBST to all the wells and incubate for 30 min. @ RT.
h) repeat step b
i) add 100 ul of enzyme substrate solution to all wells and monitor the color development (visually or instrumentally).
I hope that this protocol will be useful, but if there is anything else that we can do for you please let me know and I'll be happy to help.

Read More

Answer

Thank you for your enquiry. I am afraid that there is no information on which surface protein that this product detects. The originator of the product informed us that the immunogen for this ab68451 is a mixture of all antigenic serotypes of Escherichia coli. It reacts with all "O" and "K" antigenic serotypes of E. coli. Since this antibody is unabsorbed, it will likely cross-react with related Enterobacteriaceae. I sincerely apologize that we seem to had a datasheet error. This product has been successfully used in ELISA applications but unfortunately has yet to be tested in ICC/IF, IHC-Fr, and WB as previously stated on the datasheet. I have removed these applications from the datasheet but please rest assure that this does not mean that the product will not work in those applications, just that we have not tested them and will need to be ascertained by our laboratory or the end user. All of our products are guaranteed to work in species and application as stated on the datasheet. Should you do not get expected results, then please inform us within the guarantee period of 120 days. We will help troubleshoot the experiment and if the product is indeed faulty, we will be more than happy to offer a free replacement or refund. I hope our Abpromise guarantee will give you the assurance you need to use this product in your research.

Read More

11-12 of 12 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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