Overview

  • Product name

    Anti-E. coli antibody (Biotin)
    See all E. coli primary antibodies
  • Description

    Rabbit polyclonal to E. coli (Biotin)
  • Host species

    Rabbit
  • Conjugation

    Biotin
  • Specificity

    ab68451 reacts with E. coli in bacterial and infected tissue samples.
  • Tested applications

    Suitable for: ELISAmore details
  • Species reactivity

    Reacts with: Escherichia coli
  • Immunogen

    O and K antigenic serotypes (All types) of Escherichia coli.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab68451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.

Target

  • Relevance

    Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.
  • Alternative names

    • Escherichia coli antibody

References

This product has been referenced in:

  • Dua P  et al. Cell-SELEX Based Identification of an RNA Aptamer for Escherichia coli and Its Use in Various Detection Formats. Mol Cells 39:807-813 (2016). Functional Studies . Read more (PubMed: 27871171) »
  • Biswas B  et al. Uropathogenic Escherichia coli (UPEC) induced antimicrobial gene expression in the male reproductive tract of rat: evaluation of the potential of Defensin 21 to limit infection. Andrology 3:368-75 (2015). ICC/IF . Read more (PubMed: 25675950) »
See all 2 Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrat buffer ph6
Permeabilization
No
Specification
Liver
Blocking step
CAS as blocking agent for 10 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde

Shmuel Cohen

Verified customer

Submitted May 13 2019

Application
Flow Cytometry
Sample
Escherichia coli Cell (Escherichia Coli trapping using magnetic beads der)
Permeabilization
No
Specification
Escherichia Coli trapping using magnetic beads der
Preparation
Cell harvesting/tissue preparation method: magentic streptavidin-coated beads labeled with ab68451 (biotin) to trap E. Coli from dilutions after LB culture
Sample buffer: Phosphate Buffer Saline, pH=7.4 [0.01 M with 27 mM KCl, 137 mM NaCl]
Fixation
on magnetic streptavidin beads

Cedric Hurth

Verified customer

Submitted Apr 07 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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