Question (54771) | Anti-E. coli antibody (Biotin) (ab68451)

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Question


I am now providing you with some background information regarding the intended use of your antibody and the related procedures.The project involves use of paramagnetic beads onto which various types of cells, including E. coli, yeast and mammalian cells may be attached in studies on an electrowetting-based microfluidics platform. Ideally, activated beads should have the capacity to interact with cell surface structures and thereby pulling the cells down. Detection of cell attachment involves use of a very sensitive luminescence-based assay, carried out on the platform. This procedure has been successfully applied for yeast and mammalian cells. In fact in one of the procedures an Abcam antibody (ab19498) has been successfully utilized for yeast which seems to work better by initial attachment to the cells followed by incubation with beads (protein A). In other assays, bioactivated beads have been seen to have the capacity to pull down cells from cell suspensions very efficiently. At this time, we are most interested in having an antibody for "EcNR2", an E. coli strain which we have obtained from Addgene( http://www.addgene.org/26931/).
I hope you will be able to provide me with a sample of an E. coli antibody which will be successfully employed in our procedures.
Thank you for your cooperation.
Best wishes,

Answer

Thank you very much for your reply and for sending the description of the assay in which you will be using the E. coli antibody.

We have not tested any of our E. coli antibodies in a similar assay, so there isn't one in particular that we know will work in this assay. I think that the unconjugated antibody used with Protein A/G beads would be the best protocol to try since it has worked with other antibodies and cells, however we do not have an E. coli antibody that was raised against whole intact cells like the S. cerevisiae antibody ab19498 was. As previously mentioned, if you would like to submit a review of ab68451 in this assay, then I can provide you with a discount code that can be redeemed for one free full size antibody. So, you will be able to try a different E. coli antibody at no charge. The goat polyclonal antibodies ab13627, ab48416,and ab25823 are the only unconjugated, polyclonal E. coli antibodies that we currently sell, so you may want to try one of these. When using a goat antibody, Protein G beads will need to be substituted for the Protein A beads.

If you would like to accept this offer, please let me know and I will set-up the discount code for you. Please let me know if you have any questions or need anything else at this time.

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