Question (57181) | Anti-E. coli antibody (Biotin) (ab68451)

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Question


Thank you for your email. Would you please share the information I have provided regarding the assay involving the E. coli antibody with your colleagues here and in the U.K. and found out for me if there is any preference? I do realize none of the antibodies has been tested in an assay similar to the one I have described but there may be a reasonable guess as to which of these may work better, or may have a greater chance for success.
Please do take your time.
Thank you so much for all your help!
Best regards,

Answer

Thank you so much for your patience while I have worked on this enquiry.

I've looked through the literature, data on our E.coli antibodies, and also discussed with my colleagues, and I think that the unconjugated version of ab20640 will be the best option for isolating E.coli as you describe. The immunogen was a mixture of mostly intact bacteria of various serotypes, and the rabbit IgG will have a higher affinity for Protein A compared to the goat antibodies, which haveonly a moderate affinity for protein G. I'm currently working to add this unconjugated antibody to our catalog, and I'll let you know as soon as this is done. I expect it will be donesoon.

Thank you again for your patience, and please let me know if you have any questions or need anything else in the meantime.

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