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Thank you for your email. Would you please share the information I have provided regarding the assay involving the E. coli antibody with your colleagues here and in the U.K. and found out for me if there is any preference? I do realize none of the antibodies has been tested in an assay similar to the one I have described but there may be a reasonable guess as to which of these may work better, or may have a greater chance for success.
Please do take your time.
Thank you so much for all your help!
Asked on Aug 08 2012
Thank you so much for your patience while I have worked on this enquiry.
I've looked through the literature, data on our E.coli antibodies, and also discussed with my colleagues, and I think that the unconjugated version of ab20640 will be the best option for isolating E.coli as you describe. The immunogen was a mixture of mostly intact bacteria of various serotypes, and the rabbit IgG will have a higher affinity for Protein A compared to the goat antibodies, which haveonly a moderate affinity for protein G. I'm currently working to add this unconjugated antibody to our catalog, and I'll let you know as soon as this is done. I expect it will be donesoon.
Thank you again for your patience, and please let me know if you have any questions or need anything else in the meantime.
Answered on Aug 08 2012