Key features and details
- Rabbit polyclonal to E. coli (FITC)
- Suitable for: ICC/IF
- Reacts with: Escherichia coli
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
Product nameAnti-E. coli antibody (FITC)
See all E. coli primary antibodies
DescriptionRabbit polyclonal to E. coli (FITC)
ConjugationFITC. Ex: 493nm, Em: 528nm
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Escherichia coli
Tissue/ cell preparation (Escherichia coli, all serotypes). A mixed culture of E. coli strains (O157:H7, O20, O125, O55, O111 & K12), intact plus lysed, non-denatured, was gamma-inactivated and used directly as the immunogen.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.20
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab30522 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/25. Acetone fixation of the antigen is recommended.|
RelevanceEscherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.
- Escherichia coli antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab30522 has been referenced in 4 publications.
- Saxena A et al. Absence of the NOD2 protein renders epithelia more susceptible to barrier dysfunction due to mitochondrial dysfunction. Am J Physiol Gastrointest Liver Physiol 313:G26-G38 (2017). PubMed: 28450277
- Pazos-Perez N et al. Ultrasensitive multiplex optical quantification of bacteria in large samples of biofluids. Sci Rep 6:29014 (2016). PubMed: 27364357
- Golberg A et al. Cloud-enabled microscopy and droplet microfluidic platform for specific detection of Escherichia coli in water. PLoS One 9:e86341 (2014). PubMed: 24475107
- Heinze BC & Yoon JY Nanoparticle immunoagglutination Rayleigh scatter assay to complement microparticle immunoagglutination Mie scatter assay in a microfluidic device. Colloids Surf B Biointerfaces 85:168-73 (2011). PubMed: 21411297