Overview

  • Product name
    Anti-E. coli antibody (FITC)
    See all E. coli primary antibodies
  • Description
    Rabbit polyclonal to E. coli (FITC)
  • Host species
    Rabbit
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: ICC/IFmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Tissue/ cell preparation (Escherichia coli, all serotypes). A mixed culture of E. coli strains (O157:H7, O20, O125, O55, O111 & K12), intact plus lysed, non-denatured, was gamma-inactivated and used directly as the immunogen.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 40% Glycerol, 1% BSA, PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab30522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/25. Acetone fixation of the antigen is recommended.

Target

  • Relevance
    Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.
  • Alternative names
    • Escherichia coli antibody

References

This product has been referenced in:
  • Pazos-Perez N  et al. Ultrasensitive multiplex optical quantification of bacteria in large samples of biofluids. Sci Rep 6:29014 (2016). Read more (PubMed: 27364357) »
  • Golberg A  et al. Cloud-enabled microscopy and droplet microfluidic platform for specific detection of Escherichia coli in water. PLoS One 9:e86341 (2014). Read more (PubMed: 24475107) »
See all 3 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Flow Cytometry
Sample
Escherichia coli Cell (Planktonic)
Permeabilization
Yes - acetone
Specification
Planktonic
Fixation
Acetone

Abcam user community

Verified customer

Submitted Aug 17 2015

Answer

Thank you for your enquiry.

It might be possible for a FITC-conjugated IgG to still be able to bind to Protein A as well, primarily due to the fact that FITC conjugation to IgG and Protein A binding to IgG occur at different sites within the IgG molecule and utilize different modes of attachment. FITC binds to IgG via primary amines which are distributed in both the Fab and Fc regions. On the other hand, Protein A binding to IgG is confined to the Fc region; this non-covalent interaction involves the participation of carbohydrate (oligosaccharides) moieties attached to particular amino acids (e.g Asn) in the Fc region and the amino acid residues of Protein A.


Based on these considerations, it ispossible for IgG-FITC to still be conjugated to Protein A. However, there is also the bulk of the molecules to consider. Wedo not have an exact ratio of FITC to IgG (typical ratio will be 3 to 10) and we do not know exactly which lysine residues are conjugated, or more importantly, how many of the ˜15 lysine residues in the Fc region of rabbit IgG are conjugated to FITC, which can possibly contribute some steiric hindrance effects and affect binding of the Protein A to the Fc region. One Protein A molecule is typically able to bind two or more unconjugated IgG molecules; bulkier IgG may reduce this binding.


I hope this information will be helpful. Please note that the E. coli antibody (FITC) antibody has regrettably not been tested and guaranteed for this particular application that the customer would like to use.


If you have any further questions, please do not hesitate to contact us.

Read More

Answer

Thank you for your patience. I can now confirm that ab30522 also cross-reacts with the DH5a strain of E.coli. I hope this information is helpful for your customer.

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Escherichia coli Cell (pure culture)
Specification
pure culture

Abcam user community

Verified customer

Submitted May 09 2007

Answer

Thank you for your patience. Regarding ab30522 (E.coli antibody (FITC)), a mixed culture of E. coli strains was gamma-inactivated and used directly as the immunogen. Although the concentration has not been determined, there is a dilution recommendation (IF: 1/25. Acetone fixation of the antigen is recommended). I hope this information helps. Please don't hesitate to contact us again if you have further questions.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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