Key features and details
- Rabbit polyclonal to E. coli (HRP)
- Suitable for: ELISA, ICC/IF
- Reacts with: Escherichia coli
- Conjugation: HRP
- Isotype: IgG
Product nameAnti-E. coli antibody (HRP)
See all E. coli primary antibodies
DescriptionRabbit polyclonal to E. coli (HRP)
SpecificityAll O and K antigenic serotypes of Escherichia coli. Antiserum is not absorbed and does react with related Enterobacteriaceae.
Tested applicationsSuitable for: ELISA, ICC/IFmore details
Species reactivityReacts with: Escherichia coli
Mixture of E.coli serotypes
Will remove E. coli proteins from recombinant preparations.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.002% Thimerosal (merthiolate)
Constituents: PBS, 1% BSA
Concentration information loading...
PurityProtein A purified
Primary antibody notesWill remove E. coli proteins from recombinant preparations.
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Our Abpromise guarantee covers the use of ab20425 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Against serotypes O157:H7, O20, O125, O55, O111 and K12.
|ICC/IF||Use at an assay dependent concentration.|
RelevanceEscherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.
- Escherichia coli antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab20425 has been referenced in 6 publications.
- Sanvicens N et al. Quantum dot-based array for sensitive detection of Escherichia coli. Anal Bioanal Chem 399:2755-62 (2011). PubMed: 21249344
- Baldrich E et al. Self-assembled monolayers as a base for immunofunctionalisation: unequal performance for protein and bacteria detection. Anal Bioanal Chem 390:1557-62 (2008). PubMed: 18256810
- Baldrich E et al. Gold immuno-functionalisation via self-assembled monolayers: study of critical parameters and comparative performance for protein and bacteria detection. J Immunol Methods 336:203-12 (2008). ELISA ; Escherichia coli . PubMed: 18534611
- Laczka O et al. Immunofunctionalisation of gold transducers for bacterial detection by physisorption. Anal Bioanal Chem 391:2825-35 (2008). ELISA ; Escherichia coli . PubMed: 18553187
- Lin YH et al. Disposable amperometric immunosensing strips fabricated by Au nanoparticles-modified screen-printed carbon electrodes for the detection of foodborne pathogen Escherichia coli O157:H7. Biosens Bioelectron 23:1832-7 (2008). PubMed: 18424027
- Laczka O et al. Detection of Escherichia coli and Salmonella typhimurium using interdigitated microelectrode capacitive immunosensors: the importance of transducer geometry. Anal Chem 80:7239-47 (2008). PubMed: 18771278