Overview

  • Product name

    Anti-E. coli antibody (HRP)
    See all E. coli primary antibodies
  • Description

    Rabbit polyclonal to E. coli (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: IHC-Fr, WB, ELISAmore details
  • Species reactivity

    Reacts with: Escherichia coli
  • Immunogen

    O and K antigenic serotypes (All types) of Escherichia coli.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.002% Thimerosal (merthiolate)
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab68450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent dilution.
WB 1/20.
ELISA 1/200.

Target

  • Relevance

    Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.
  • Alternative names

    • Escherichia coli antibody

References

This product has been referenced in:

  • Wang C  et al. Detection of extremely low concentration waterborne pathogen using a multiplexing self-referencing SERS microfluidic biosensor. J Biol Eng 11:9 (2017). Read more (PubMed: 28289439) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Question
Answer

The immungen is a mixture of intact and lysed bacteria of various E. coli strains, so the antibody will likely detect surface antigens in addition to intracellular antigens.

Read More
Application
ELISA
Sample
Mouse Serum (Intestinal fluid)
Specification
Intestinal fluid
Type
Sandwich (Detection)
Blocking step
Pierce No Protein Blocking Buffer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Sarah Hoyle

Verified customer

Submitted Nov 12 2013

Answer

Thank you for contacting us.

I have attached the list of antibodies we have along with details. Please check.

I am sorry I am unable to confirm the species cross reactivity of the these antibodies other than waht is given on the datasheet.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Answer

Thank you for contacting us.

ab41181 is anti Strep agalactiae, it will weakly cross react with Strep. dysgalactiae.

ab41203 is anti Strep agalactiae, it will weakly cross react with Strep. dysgalactiae.

ab19980 is an anti Enterococcus antibody

ab68450, ab69185 and ab68451 are anti E. Coli antibodies.

ab69468, ab20947, ab69467 and ab65926 are the Klebsiella spp specific antibodies.

We do not have anti Strep. uberis antibodies.

Please check our new western blot range:

https://www.abcam.com/index.html?c=5634

https://www.abcam.com/index.html?pageconfig=productmap&cl=918

https://www.abcam.com/index.html?pageconfig=resource&rid=12886

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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