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    e-coli-hcp-elisa-kit-host-cell-protein-ab240997.pdf

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Microbiology Organism Bacteria Gram negative Bacteria Escherichia coli
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E. coli HCP ELISA Kit (host cell protein) (ab240997)

  • Datasheet
  • SDS
  • Protocol Booklet
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Key features and details

  • Sensitivity: 30 ng/ml
  • Range: 3 ng/ml - 810 ng/ml
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)

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Overview

  • Product name

    E. coli HCP ELISA Kit (host cell protein)
  • Detection method

    Colorimetric
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    30 ng/ml
  • Range

    3 ng/ml - 810 ng/ml
  • Assay time

    3h 00m
  • Assay duration

    Multiple steps standard assay
  • Product overview

    E. coli HCP ELISA Kit (host cell protein) (ab240997) is designed to quantitatively measure host cell protein (HCP) contamination in bulk products expressed in E. coli expression systems.


    The provided 96-well plate has been pre-coated with Rabbit anti-E. coli HCP antibodies to capture E. coli proteins from biological samples. Following an incubation to allow capture of the E. coli protein by the antibodies on the plate, a second anti- E. coli HCP antibody, conjugated with biotin, is added and incubated to allow it to bind to the captured E. coli proteins. After incubation, the plate is washed and a Streptavidin-HRP conjugate is added and incubated for 30 min. The Streptavidin-HRP conjugate will be captured by any biotin labeled antibody bound to the plate. Following a wash step to remove unbound conjugate, TMB substrate is added and is converted by the captured HRP to a colored product in proportion to the amount of HCP bound to the plate. After a short incubation to allow color development, the reaction is stopped and the intensity of the generated color is detected in a spectrophotometer plate reader capable of measuring 450nm wavelength.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    E. coli Protein Standard 1 x 600µl
    10X PBST 1 x 30ml
    5X Dilution Buffer 1 x 15ml
    Coated Clear 96 Well Plates 1 unit
    Plate Sealer 1 unit
    Reporting Antibody 1 x 150µl
    Stop Solution 1 x 15ml
    Streptavidin-HRP Conjugate 1 x 400µl
    TMB Substrate 1 x 15ml
  • Research areas

    • Microbiology
    • Organism
    • Bacteria
    • Gram negative Bacteria
    • Escherichia coli
  • Relevance

    Escherichia coli is a bacterium that is commonly found in the lower intestine of warm-blooded animals.
  • Alternative names

    • Escherichia coli

Associated products

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab240997? Please let us know so that we can cite the reference in this datasheet.

    ab240997 has not yet been referenced specifically in any publications.

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