E. coli HCP ELISA Kit (host cell protein) (ab240997)
- Datasheet
- References
- Protocols
Overview
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Product name
E. coli HCP ELISA Kit (host cell protein) -
Detection method
Colorimetric -
Assay type
Sandwich (quantitative) -
Sensitivity
30 ng/ml -
Range
3 ng/ml - 810 ng/ml -
Assay time
3h 00m -
Assay duration
Multiple steps standard assay -
Product overview
E. coli HCP ELISA Kit (host cell protein) (ab240997) is designed to quantitatively measure host cell protein (HCP) contamination in bulk products expressed in E. coli expression systems.
The provided 96-well plate has been pre-coated with Rabbit anti-E. coli HCP antibodies to capture E. coli proteins from biological samples. Following an incubation to allow capture of the E. coli protein by the antibodies on the plate, a second anti- E. coli HCP antibody, conjugated with biotin, is added and incubated to allow it to bind to the captured E. coli proteins. After incubation, the plate is washed and a Streptavidin-HRP conjugate is added and incubated for 30 min. The Streptavidin-HRP conjugate will be captured by any biotin labeled antibody bound to the plate. Following a wash step to remove unbound conjugate, TMB substrate is added and is converted by the captured HRP to a colored product in proportion to the amount of HCP bound to the plate. After a short incubation to allow color development, the reaction is stopped and the intensity of the generated color is detected in a spectrophotometer plate reader capable of measuring 450nm wavelength.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests E. coli Protein Standard 1 x 600µl 10X PBST 1 x 30ml 5X Dilution Buffer 1 x 15ml Coated Clear 96 Well Plates 1 unit Plate Sealer 1 unit Reporting Antibody 1 x 150µl Stop Solution 1 x 15ml Streptavidin-HRP Conjugate 1 x 400µl TMB Substrate 1 x 15ml -
Research areas
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Relevance
Escherichia coli is a bacterium that is commonly found in the lower intestine of warm-blooded animals. -
Alternative names
- Escherichia coli
Protocols
References
ab240997 has not yet been referenced specifically in any publications.
