Anti-E. coli J5 LPS antibody (ab54089)

Submit an Abreview Q&A (2)

Overview

  • Product name
    Anti-E. coli J5 LPS antibody
  • Description
    Sheep polyclonal to E. coli J5 LPS
  • Host species
    Sheep
  • Specificity
    ab54089 recognises the J5 mutant of E. coli 0:111 which lacks the enzyme uridine diphosphate glucose 4-epimerase and therefore produces an incomplete LPS, deficient in galactose and all the sugars distal to the central polymers. The 55 mutant has no O-specific chains and its endotoxin remains as the core LPS containing lipid A, N acetyl glucosamine, 2-keto-3-deoxyoctonate, heptose and glucose (a composition similar to that of the Rc strains of Salmonella).
  • Tested applications
    Suitable for: ELISAmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Heat inactivated Escherichia coli J5 (Rc mutant; ATCC)

Properties

Applications

Our Abpromise guarantee covers the use of ab54089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.

Target

  • Relevance
    LPS is a major component of the cell membrane of Gram negative bacteria, contributing greatly to the structural integrity of the bacteria, and protecting the membrane from certain kinds of chemical attack. LPS is an endotoxin, and induces a strong response from normal animal immune systems.
  • Cellular localization
    Cell Membrane
  • Alternative names
    • Escherichia coli J5 lipopolysaccharide antibody
    • Escherichia coli J5 LPS antibody

References

ab54089 has not yet been referenced specifically in any publications.

Publishing research using ab54089? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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2 reviews or Q&A

10
Votes: Highest First

Dear technical team,

Our customer purchased ab54089 (lot# GR67959-1).
This product is whole antiserum and there is no mention of concentration on the datasheet.
So she would like to know the concentration.
Please let me know. ...

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Thank you for your enquiry and your interest in our products.

As you may be aware, we can only provide the concentration of the IgG for affinity purified antibodies.

I would kindly advise you and your customers to read the following...

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I am looking for an antibody which can recognize both smooth and rough (ReLPS) LPS. Is it possible using the E. coli J5 LPS antibody?

Thank you for your enquiry. I have contacted the originator of this LPS antibody. They have confirmed it has been tested successfully with rough LPS. Unfortunately, it has not been tested on smooth LPS so we are unable to state whether or not it wi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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