Anti-E. coli J5 LPS antibody (ab54089)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-E. coli J5 LPS antibody -
Description
Sheep polyclonal to E. coli J5 LPS -
Host species
Sheep -
Specificity
ab54089 recognises the J5 mutant of E. coli 0:111 which lacks the enzyme uridine diphosphate glucose 4-epimerase and therefore produces an incomplete LPS, deficient in galactose and all the sugars distal to the central polymers. The 55 mutant has no O-specific chains and its endotoxin remains as the core LPS containing lipid A, N acetyl glucosamine, 2-keto-3-deoxyoctonate, heptose and glucose (a composition similar to that of the Rc strains of Salmonella). -
Tested applications
Suitable for: ELISAmore details -
Species reactivity
Reacts with: Escherichia coli -
Immunogen
Heat inactivated Escherichia coli J5 (Rc mutant; ATCC)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Whole serum -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab54089 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ELISA | Use at an assay dependent dilution. |
Target
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Relevance
LPS is a major component of the cell membrane of Gram negative bacteria, contributing greatly to the structural integrity of the bacteria, and protecting the membrane from certain kinds of chemical attack. LPS is an endotoxin, and induces a strong response from normal animal immune systems. -
Cellular localization
Cell Membrane -
Alternative names
- Escherichia coli J5 lipopolysaccharide antibody
- Escherichia coli J5 LPS antibody
References
This product has been referenced in:
- Chen H et al. An LRR-domain containing protein identified in Bathymodiolus platifrons serves as intracellular recognition receptor for the endosymbiotic methane-oxidation bacteria. Fish Shellfish Immunol 93:354-360 (2019). Read more (PubMed: 31306759) »