Anti-E. coli J5 LPS antibody (ab54089)

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Overview

  • Product name

    Anti-E. coli J5 LPS antibody

  • Description

    Sheep polyclonal to E. coli J5 LPS

  • Host species

    Sheep

  • Specificity

    ab54089 recognises the J5 mutant of E. coli 0:111 which lacks the enzyme uridine diphosphate glucose 4-epimerase and therefore produces an incomplete LPS, deficient in galactose and all the sugars distal to the central polymers. The 55 mutant has no O-specific chains and its endotoxin remains as the core LPS containing lipid A, N acetyl glucosamine, 2-keto-3-deoxyoctonate, heptose and glucose (a composition similar to that of the Rc strains of Salmonella).

  • Tested applications

    Suitable for: ELISAmore details

  • Species reactivity

    Reacts with: Escherichia coli

  • Immunogen

    Heat inactivated Escherichia coli J5 (Rc mutant; ATCC)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab54089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.

Target

  • Relevance

    LPS is a major component of the cell membrane of Gram negative bacteria, contributing greatly to the structural integrity of the bacteria, and protecting the membrane from certain kinds of chemical attack. LPS is an endotoxin, and induces a strong response from normal animal immune systems.

  • Cellular localization

    Cell Membrane

  • Alternative names

    • Escherichia coli J5 lipopolysaccharide antibody
    • Escherichia coli J5 LPS antibody

References

This product has been referenced in:

  • Chen H  et al. An LRR-domain containing protein identified in Bathymodiolus platifrons serves as intracellular recognition receptor for the endosymbiotic methane-oxidation bacteria. Fish Shellfish Immunol 93:354-360 (2019). Read more (PubMed: 31306759) »
See 1 Publication for this product

Publishing research using ab54089? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

Dear technical team,

Our customer purchased ab54089 (lot# GR67959-1).
This product is whole antiserum and there is no mention of concentration on the datasheet.
So she would like to know the concentration.
Please let me know.

Best regards

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Answer

Thank you for your enquiry and your interest in our products.

As you may be aware, we can only provide the concentration of the IgG for affinity purified antibodies.

I would kindly advise you and your customers to read the following section under the FAQ to get more understanding on the concentration of the differently purified primary antibodies:

https://www.abcam.com/index.html?pageconfig=technicalfaqs#8

If you need any further assistance in the future, please do not hesitate to contact me.

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Question

I am looking for an antibody which can recognize both smooth and rough (ReLPS) LPS. Is it possible using the E. coli J5 LPS antibody?

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Answer

Thank you for your enquiry. I have contacted the originator of this LPS antibody. They have confirmed it has been tested successfully with rough LPS. Unfortunately, it has not been tested on smooth LPS so we are unable to state whether or not it will detect smooth LPS. I hope this information is helpful to you. Should you have any further questions, please do not hesitate to contact us.

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