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I have a few questions relating to the abovementioned product number.
1) Was the animal immunised with whole cell J5 or LPS from J5 cells ? If whole cells were injected then where does the LPS specificity come from. Did the manufacturers do epitope mapping?
2) Is the antibody E coli J5 specific or does it detect across a range of E coli serotypes. Was cross reactivity studies conducted by the manufacturer and if so, are these results available ?
We are looking at purchasing a broad spectrum Anti E coli antibody and therefore clarity on these questions are important before a decision is made to purchase.
Asked on May 23 2013
Thank you for contacting us and your interest in our products.
The Anti-E. coli LPS antibody [2D7/1], ab35654, has been raised against whole E. coli cells (rather than disrupted or sonicated cells). Whilst specific epitope mapping has not been performed, the outer cell wall of E. coli is predominantly LPS and one would need to remove this before the other cell wall proteins would be exposed. Additionally, information from the developers of this clone have indicated that the antibody does indeed detect J5 LPS by ELISA. Unfortunately, cross reactivity with other E. coli proteins has not been performed so this does remain a possibility (albeit a remote one).
Aside from E. coli J5, the clone was found to be reactive to K. pneumoniae, S. sonnei and S. typhimurium. Unfortunately, it has not been tested against other E. coli serotypes and we currently do not have any empirical data to indicate which other serotypes, if any, are recognised by this clone.
I hope this has been helpful. If you have any further questions, please do not hesitate to let me know.
Answered on May 23 2013