ELISA abreview for Anti-E. coli LPS antibody [2D7/1]

Excellent
Abreviews
Application
ELISA
Sample
Mouse Serum (Intestinal fluid)
Specification
Intestinal fluid
Type
Sandwich (Capture)
Blocking step
Pierce No Protein Blocking Buffer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 100mM sodium carbonate/bicarbonate pH9.6
Dilution
1/500

Secondary antibody

Secondary antibody
None used

Additional data

Additional Notes
This antibody was used to detect E coli in fluid flushed through intestinal tissue in a sandwich ELISA. Dilutions of the fluid produced a nice dose response. Detection antibody = ab68450 anti E.coli HRP (1:200).

Sarah Hoyle

Verified customer

Submitted Nov 12 2013

Sign up