Overview

  • Product name
    Anti-E. coli O + E. coli K antibody (Biotin)
    See all E. coli O + E. coli K primary antibodies
  • Description
    Goat polyclonal to E. coli O + E. coli K (Biotin)
  • Host species
    Goat
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: ICC/IFmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Tissue/ cell preparation (Mixture of E. coli serotypes).

  • General notes

    The antibody consists of purified antibody covalently coupled with the N-Hydroxysuccinimide ester of biotin under mild conditions to give a high degree of substitution.

Properties

Applications

Our Abpromise guarantee covers the use of ab20765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
  • Application notes
    Avidin and streptavidin amplification systems for IF: Use at an assay dependent dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Escherichia coli (E. coli) are Gram negative enteric bacteria that live in the human gut. Its presence in nature is an indication of human feacal pollution. E. coli also causes human disease such as urinary tract infections and neonatal meningitis. Infection with particular enterotoxigenic strains can lead to life threatening intestinal diseases. E. coli are distinguished immunologically by serotyping based on three types of antigen: the somatic or O antigen which corresponds to terminal sugars on the cell surface lipopolysaccharide, the capsular or K antigens and the flagellar or H antigens.
    • Alternative names
      • Capsular antigen antibody
      • Escherichia coli antibody
      • Escherichia coli K antibody
      • Escherichia coli O antibody
      • K antigen antibody
      • O antigen antibody
      see all

    References

    ab20765 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    I'm glad you found the information of help.

    If you do test any of these antibodies we would be very interested to hear of the results, regardless of if they are positive or not. We like to encourage all of our customers to submit an Abreview via the online product datasheet with any data they collect with our antibodies. As a reward for doing this, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link:

    https://www.abcam.com/abreviews

    If you let me know which antibody in particular you are interested in using I may be able to provide you with a testing discount code. More information on this scheme can be found form the following link:

    www.abcam.com/collaborationdiscount

    I hope this information has been of help. If you have any further questions, please do not hesitate to ask.

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    Answer

    Thank you for contacting us and your interest in our products.

    I can now share some more information relating to the antibodies we have directed towards E. coli (O and K). We have two different antibodies directed against this bacterium, with different conjugation available:

    ab31499, unlabelled(ab33604, FITC; ab33837, HRP; ab33839, Biotin conjugated of same antibody preparation as ab31499)

    The immunogen for these antibodies is a mixture of the following E. coli strains: O157:H7, O20, O125, O55, O111 & K12. The mixture of these strains was of intact plus lysed bacteria which had not been denatured in any way. All E. coli have many surface and internal antigens in common and this antibody recognizes this large mixture and therefore reacts with most, if not all, E. coli. Theseantibodies have been tested against the strains listed above but have not been cross-absorbed and may react with related Enterobacteriaceae. These potential cross-reacting strains have not been identified.

    ab20765, ab20856 and ab21110 are Biotin, FITC and HRP conjugates of the same antibody.

    These antibodies have been raised against a mixture of E. coli serotypes.These antibodies have been tested for reactivity against the immunogen strains of E.coli but again they have not been absorbed and will most likely cross react withother related Enterobacteriaceae.



    I am sorry that in this case I was not able to be of more help. If you have any further questions, please do not hesitate to contact us again.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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