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  1. Link

    e-coli-o157-antibody-ab30521.pdf

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Microbiology Organism Bacteria Gram negative Bacteria Escherichia coli
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Anti-E. coli O157 antibody (ab30521)

  • Datasheet
  • SDS
Submit a review Q&A (2)

Product price, shipping and contact information

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Abpromise

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Key features and details

  • Goat polyclonal to E. coli O157
  • Suitable for: ELISA
  • Reacts with: Escherichia coli
  • Isotype: IgG

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Donkey Anti-Goat IgG H&L (HRP) (ab97110)

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Overview

  • Product name

    Anti-E. coli O157 antibody
    See all E. coli O157 primary antibodies
  • Description

    Goat polyclonal to E. coli O157
  • Host species

    Goat
  • Specificity

    ab30521 recognises Escherichia coli, O157:H7 (E. coli).
  • Tested applications

    Suitable for: ELISAmore details
  • Species reactivity

    Reacts with: Escherichia coli
  • Immunogen

    Tissue/ cell preparation (whole cells of Escherichia coli O157:H7)

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituents: PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Organism
    • Bacteria
    • Gram negative Bacteria
    • Escherichia coli

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab30521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent dilution.
Notes
ELISA
Use at an assay dependent dilution.

Target

  • Relevance

    Escherichia coli are Gram negative bacterium that are commonly found in the lower intestine of warm-blooded organisms (endotherms). Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigens (H type: H1-H56). The E. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenic E. coli, which are classified into the following 4 groups according to differences in the mode of pathogenicity; enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC) and enterohemorrhagic E. coli (EHEC). Although the identification of pathogenic E. coli requires verification of their pathogenicity, pathogenic E. coli often have specific serotypes; therefore, typing of the serogroup and serotype is necessary in screening pathogenic E. coli.
  • Alternative names

    • Escherichia coli O157 antibody

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab30521? Please let us know so that we can cite the reference in this datasheet.

ab30521 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
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1-2 of 2 Abreviews or Q&A

Question

Certificate of Compliance request

Read More

Abcam community

Verified customer

Asked on May 31 2012

Answer

Thank you for contacting Abcam.

Please find attached the Certificate of Compliances for the 6 antibodies that you purchased recently.

If there is anything else I can help you with, please let me know.

Read More

Abcam Scientific Support

Answered on May 31 2012

Question

Dear Sir/Madam, Do you have the deactivated E Coli O157 bacterial cell? Kindly note that we are looking for whole bacterial cells, instead of the lysate. Please advise, thank you. Regards,  

Read More

Abcam community

Verified customer

Asked on Nov 03 2011

Answer

I regret to inform you that currently we do not have the deactivated E Coli O157 bacterial cells in our catalogue. If you need any further assistance in the future, please do not hesitate to contact me.  

Read More

Abcam Scientific Support

Answered on Nov 03 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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