The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
See attached protocol.
Escherichia coli is a group of gram-negative bacilli belonging to the family Enterobacteriaceae and is considered as one of the bacteria in the human intesinal normal microbial flora. Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigen (H type: H1-H56).
The E. Coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenic E. Coli. These are classified into the following four groups according to differences in the mode of pathogenicity; enteropathogenic E. Coli (EPEC), enteroinvasive E. Coli (EIEC), enterotoxigenic E. Coli (ETEC) and enterohemorrhagic E. Coli (EHEC).
Although the identification of pathogenic E. Coli requires verification of their pathogenicity, pathogenic E. Coli have often specific serotypes; therefore, typing of the serogroup and serotype is necessary to screening pathogenic E. Coli.