Overview

  • Product name

    Anti-E. coli O78 antibody
  • Description

    Rabbit polyclonal to E. coli O78
  • Host species

    Rabbit
  • Specificity

    ab78826 is specific for E. coli O78 somatic O antigen.
  • Tested applications

    Suitable for: Agglutinationmore details
  • Species reactivity

    Reacts with: Escherichia coli
  • Immunogen

    Tissue/ cell preparation of E. coli O78 (heat inactivated).

  • Positive control

    • Antigenic suspension.
  • General notes

    Reagents should be allowed to stand at 15°C - 25°C for at least 30 minutes prior to use.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.08% Sodium azide
    Constituent: Whole serum
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab78826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Agglutination Use at an assay dependent concentration.

See attached protocol.

Target

  • Relevance

    Escherichia coli is a group of gram-negative bacilli belonging to the family Enterobacteriaceae and is considered as one of the bacteria in the human intesinal normal microbial flora. Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigen (H type: H1-H56). The E. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenic E. coli. These are classified into the following four groups according to differences in the mode of pathogenicity; enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC) and enterohemorrhagic E. coli (EHEC). Although the identification of pathogenic E.coli requires verification of their pathogenicity, pathogenic E. coli often have specific serotypes; therefore, typing of the serogroup and serotype is necessary in screening pathogenic E. coli.
  • Alternative names

    • Escherichia coli O78 antibody

References

ab78826 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Escherichia coli Cell lysate - whole cell (E. coli H10407)
Loading amount
2.5 µg
Specification
E. coli H10407
Gel Running Conditions
Reduced Denaturing (4 to 12%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Jan 11 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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