Product nameAnti-E-Syt1 antibody
See all E-Syt1 primary antibodies
DescriptionRabbit polyclonal to E-Syt1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human E-Syt1 aa 1054-1104.
Database link: NP_056107.1
- HeLa, 293T and Jurkat whole cell lysates.
Previously labelled as FAM62A.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab118805 was affinity purified using an epitope specific to E-Syt1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab118805 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 123 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionMay play a role as calcium-regulated intrinsic membrane protein.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the extended synaptotagmin family.
Contains 5 C2 domains.
Cellular localizationMembrane. Localizes to intracellular membranes.
- Information by UniProt
- E Syt1 antibody
- E-Syt1 antibody
- Esyt1 antibody
All lanes : Anti-E-Syt1 antibody (ab118805) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 123 kDa
Exposure time: 3 minutes
Detection of E-Syt1 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab118805 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 3 minutes.
ab118805 has not yet been referenced specifically in any publications.