Overview

  • Product name

  • Description

    Rabbit polyclonal to E-Syt1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human E-Syt1 aa 1054-1104.
    Database link: NP_056107.1

  • Positive control

    • HeLa, 293T and Jurkat whole cell lysates.
  • General notes

    Previously labelled as FAM62A.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab118805 was affinity purified using an epitope specific to E-Syt1 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab118805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 123 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    May play a role as calcium-regulated intrinsic membrane protein.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the extended synaptotagmin family.
    Contains 5 C2 domains.
  • Cellular localization

    Membrane. Localizes to intracellular membranes.
  • Information by UniProt
  • Database links

  • Alternative names

    • E Syt1 antibody
    • E-Syt1 antibody
    • Esyt1 antibody
    • ESYT1_HUMAN antibody
    • Extended synaptotagmin 1 antibody
    • Extended synaptotagmin like protein 1 antibody
    • Extended synaptotagmin-1 antibody
    • FAM62A antibody
    • Family with sequence similarity 62 (C2 domain containing) member A antibody
    • Family with sequence similarity 62 member A antibody
    • KIAA0747 antibody
    • MBC2 antibody
    • Membrane bound C2 domain containing protein antibody
    • Membrane-bound C2 domain-containing protein antibody
    • Protein FAM62A antibody
    see all

Images

  • All lanes : Anti-E-Syt1 antibody (ab118805) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 123 kDa


    Exposure time: 3 minutes
  • Detection of E-Syt1 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab118805 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
    Detection: Chemiluminescence with an exposure time of 3 minutes.

References

ab118805 has not yet been referenced specifically in any publications.

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