Anti-E tag antibody (ab95868)
Key features and details
- Goat polyclonal to E tag
- Suitable for: WB
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-E tag antibody
See all E tag primary antibodies -
Description
Goat polyclonal to E tag -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to E tag conjugated to keyhole limpet haemocyanin.
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Positive control
- WB: E. coli whole cell lysate expressing a multi-tag fusion protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95868 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. For colorimetric techniques use at a dilution of 1/1000 - 1/10000 and for chemiluminescent techniques use at a dilution of 1/1000 - 1/30000.
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Notes |
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WB
Use at an assay dependent concentration. For colorimetric techniques use at a dilution of 1/1000 - 1/10000 and for chemiluminescent techniques use at a dilution of 1/1000 - 1/30000. |
Target
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Relevance
E tag is a peptide epitope tag, sequence GAPVPYPDPLEPR. -
Alternative names
- E epitope tag antibody
- GAPVPYPDPLEPR epitope tag antibody
- GAPVPYPDPLEPR tag antibody
Images
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All lanes : Anti-E tag antibody (ab95868) at 0.04 µg/ml
Lane 1 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.2 µg
Lane 2 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.1 µg
Lane 3 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.05 µg
Exposure time: 30 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab95868 has been referenced in 2 publications.
- Mont S et al. Accumulation of isolevuglandin-modified protein in normal and fibrotic lung. Sci Rep 6:24919 (2016). IF . PubMed: 27118599
- Ouchi T et al. Langerhans cell antigen capture through tight junctions confers preemptive immunity in experimental staphylococcal scalded skin syndrome. J Exp Med 208:2607-13 (2011). WB . PubMed: 22143886