Overview

  • Product name
    Anti-E tag antibody (Agarose)
    See all E tag primary antibodies
  • Description
    Goat polyclonal to E tag (Agarose)
  • Host species
    Goat
  • Conjugation
    Agarose
  • Tested applications
    Suitable for: IPmore details
  • Immunogen

    Synthetic peptide:

    GAPVPYPDPLEPR

    (E tag) conjugated to KLH.

  • General notes

    100 ug antibody/200 ul gel. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG, before coupling to agarose beads. Goat anti-human E Tag affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method.

Properties

Applications

Our Abpromise guarantee covers the use of ab19368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.

Target

  • Relevance
    E tag is a peptide epitope tag, sequence GAPVPYPDPLEPR.
  • Alternative names
    • E epitope tag antibody
    • GAPVPYPDPLEPR epitope tag antibody
    • GAPVPYPDPLEPR tag antibody

References

This product has been referenced in:
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

Thank you for your enquiry. I can confirm that these 2 products are the same, just that ab19400 was HRP conjugated, and ab19368 was immobilized to agarose.

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Question
Answer

Thank you for your enquiry. Yes, this product can be reused after IP. I have also attached the protocol below for your reference. Purification Procedure using Antibody on Agarose Beads 1. The agarose beads with the antibody bound to them are ready for use when you receive them. 2. Place the beads in a column and equilibrate with the appropriate buffer, usually two to three column volumes of PBS or TBS. 3. Pass the media or serum that contains the protein of interest through the column. 4. Wash the beads with several volumes of the equilibrating buffer. 5. Wash the column with 2 times the volume of the media passed originally in step 3 of a solution contain 1 M NaCl and buffer of your choice, ie. phosphate or tris. 6. Rinse the salt through the column with at least 2 column volumes of equilibrating buffer 7. Elute the desired protein with one of several buffers of your choice that is compatible with the activity of your protein: A. An acid buffer down to pH of 2.5, i.e. 5% acitic acid or glycine HCl. B. 3 or 4 M MgCl2 (magnesium chloride). C. 6 or 8 M Urea. D. 4 or 6 M Guanidine. E. 3 to 4 M Potassium Isothiocyantate. 8. Pass several volumes of equilibrating buffer over beads to remove elution buffer. 9. Neutralize (in the case of acidic eluation) with tris base and/or dialyze against a desired buffer. 10. Store the agarose-antibody beads in a buffer containing a preservative i.e. 0.1% sodium azide. They may be use many times. Note: The agarose that we use is Sterogene’s Uniflow4

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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