Key features and details
- Rabbit polyclonal to E1 Ubiquitin Activating Enzyme 1/UBA1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody
See all E1 Ubiquitin Activating Enzyme 1/UBA1 primary antibodies
DescriptionRabbit polyclonal to E1 Ubiquitin Activating Enzyme 1/UBA1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow
Recombinant fragment within Human E1 Ubiquitin Activating Enzyme 1/UBA1 (internal sequence). The exact sequence is proprietary.
Database link: P22314
- WB: IMR32 and U-87 MG whole cell lysate.
This product was previously labelled as E1 Ubiquitin Activating Enzyme
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228793 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/20000. Predicted molecular weight: 118 kDa.|
FunctionActivates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.
PathwayProtein modification; protein ubiquitination.
Involvement in diseaseDefects in UBA1 are the cause of spinal muscular atrophy X-linked type 2 (SMAX2) [MIM:301830]; also known as X-linked lethal infantile spinal muscular atrophy, distal X-linked arthrogryposis multiplex congenita or X-linked arthrogryposis type 1 (AMCX1). Spinal muscular atrophy refers to a group of neuromuscular disorders characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy. SMAX2 is a lethal infantile form presenting with hypotonia, areflexia, and multiple congenital contractures.
Sequence similaritiesBelongs to the ubiquitin-activating E1 family.
- Information by UniProt
- A1S9 antibody
- A1S9 protein antibody
- A1S9T and BN75 temperature sensitivity complementing antibody
All lanes : Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (ab228793) at 1/10000 dilution
Lane 1 : IMR32 (human neuroblast cell line) whole cell lysate
Lane 2 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 118 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228793 has not yet been referenced specifically in any publications.