Product nameAnti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody
See all E1 Ubiquitin Activating Enzyme 1/UBA1 primary antibodies
DescriptionRabbit polyclonal to E1 Ubiquitin Activating Enzyme 1/UBA1
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Cow
Synthetic peptide aa 1-50. The exact sequence is proprietary.
Database link: P22314
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates; IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264179 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000.|
FunctionActivates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.
PathwayProtein modification; protein ubiquitination.
Involvement in diseaseDefects in UBA1 are the cause of spinal muscular atrophy X-linked type 2 (SMAX2) [MIM:301830]; also known as X-linked lethal infantile spinal muscular atrophy, distal X-linked arthrogryposis multiplex congenita or X-linked arthrogryposis type 1 (AMCX1). Spinal muscular atrophy refers to a group of neuromuscular disorders characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy. SMAX2 is a lethal infantile form presenting with hypotonia, areflexia, and multiple congenital contractures.
Sequence similaritiesBelongs to the ubiquitin-activating E1 family.
- Information by UniProt
- A1S9 antibody
- A1S9 protein antibody
- A1S9T and BN75 temperature sensitivity complementing antibody
All lanes : Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (ab264179) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T whole cell lysate at 50 µg
Lane 5 : NIH/3T3 whole cell lysate at 50 µg
All lanes : Goat anti-Rabbit Light Chain HRP Conjugate
Exposure time: 30 seconds
UBA1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (20% of IP loaded) using ab264179 at 3 µg/mg lysate. Western blot was performed on the immunprecipitate using ab264179 at 1 µg/ml.
Lane 1: ab264179 in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.
Detected with chemilimuniscence with exposure time of 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264179 has not yet been referenced specifically in any publications.