Overview

  • Product name

    Anti-E1B-AP5 antibody [EPR2632(2)]
    See all E1B-AP5 primary antibodies
  • Description

    Rabbit monoclonal [EPR2632(2)] to E1B-AP5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human E1B-AP5 (N terminal). The exact sequence is proprietary.

  • Positive control

    • Raji, Jurkat, HeLa and MCF7 cell lysates; MCF7 cells
  • General notes

     

     

    Previously labelled as HNRPUL1. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab137016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Acts as a basic transcriptional regulator. Represses basic transcription driven by several virus and cellular promoters. When associated with BRD7, activates transcription of glucocorticoid-responsive promoter in the absence of ligand-stimulation. Plays also a role in mRNA processing and transport. Binds avidly to poly(G) and poly(C) RNA homopolymers in vitro.
    • Sequence similarities

      Contains 1 B30.2/SPRY domain.
      Contains 1 SAP domain.
    • Domain

      The RGG-box domain is methylated.
    • Post-translational
      modifications

      Methylated.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Adenovirus early region 1B associated protein 5 antibody
      • Adenovirus early region 1B-associated protein 5 antibody
      • E130317O14Rik antibody
      • E1B 55 kDa associated protein 5 antibody
      • E1B AP5 antibody
      • E1B-55 kDa-associated protein 5 antibody
      • E1B-AP5 antibody
      • E1BAP5 antibody
      • fb53d10 antibody
      • fk45c03 antibody
      • FLJ12944 antibody
      • Heterogeneous nuclear ribonucleoprotein U-like protein 1 antibody
      • HNRL1_HUMAN antibody
      • Hnrnpul1 antibody
      • HNRPUL1 antibody
      • MGC36621 antibody
      • MGC78330 antibody
      • wu:fb53d10 antibody
      • wu:fk45c03 antibody
      • zgc:85971 antibody
      see all

    Images

    • All lanes : Anti-E1B-AP5 antibody [EPR2632(2)] (ab137016) at 1/1000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 42 kDa

    • Flow cytometric analysis of permeabilized MCF7 cells lablling E1B-AP5 with ab137016 at 1/10 dilution (red). Rabbit IgG negative control is green.

    References

    ab137016 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab137016.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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