Product nameAnti-E1B-AP5 antibody [EPR2632(2)]
See all E1B-AP5 primary antibodies
DescriptionRabbit monoclonal [EPR2632(2)] to E1B-AP5
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human E1B-AP5 (N terminal). The exact sequence is proprietary.
- Raji, Jurkat, HeLa and MCF7 cell lysates; MCF7 cells
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab137016 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 42 kDa.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionActs as a basic transcriptional regulator. Represses basic transcription driven by several virus and cellular promoters. When associated with BRD7, activates transcription of glucocorticoid-responsive promoter in the absence of ligand-stimulation. Plays also a role in mRNA processing and transport. Binds avidly to poly(G) and poly(C) RNA homopolymers in vitro.
Sequence similaritiesContains 1 B30.2/SPRY domain.
Contains 1 SAP domain.
DomainThe RGG-box domain is methylated.
- Information by UniProt
- Adenovirus early region 1B associated protein 5 antibody
- Adenovirus early region 1B-associated protein 5 antibody
- E130317O14Rik antibody
All lanes : Anti-E1B-AP5 antibody [EPR2632(2)] (ab137016) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 42 kDa
Flow cytometric analysis of permeabilized MCF7 cells lablling E1B-AP5 with ab137016 at 1/10 dilution (red). Rabbit IgG negative control is green.
ab137016 has not yet been referenced specifically in any publications.