Overview

  • Product name

    Anti-E2F1 antibody [8G9]
    See all E2F1 primary antibodies
  • Description

    Mouse monoclonal [8G9] to E2F1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Purified recombinant fragment of Human E2F1 (amino acids 69-223) expressed in E. Coli.

  • Positive control

    • Human E2F1 recombinant protein; HeLa, SK-N-SH and NIH3T3 cell lysates; HeLa cells; esophageal and rectum cancer tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab135251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 47 kDa.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.
  • Sequence similarities

    Belongs to the E2F/DP family.
  • Post-translational
    modifications

    Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
    Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Dmel\CG6376 antibody
    • Dmel_CG6376 antibody
    • drosE2F1 antibody
    • E(Sev-CycE)3A antibody
    • E(var)3-93E antibody
    • E2-promoter binding facto antibody
    • E2F 1 antibody
    • E2F transcription factor 1 antibody
    • E2F-1 antibody
    • E2f-PA antibody
    • E2f-PB antibody
    • E2f-PC antibody
    • E2F1 antibody
    • E2f1 E2F transcription factor 1 antibody
    • E2F1_HUMAN antibody
    • Evar(3)164 antibody
    • KIAA4009 antibody
    • l(3)07172 antibody
    • l(3)j3B1 antibody
    • l(3)j3C2 antibody
    • l(3)rM729 antibody
    • mKIAA4009 antibody
    • OTTHUMP00000030661 antibody
    • PBR3 antibody
    • PRB binding protein E2F 1 antibody
    • PRB-binding protein E2F-1 antibody
    • RBAP 1 antibody
    • RBAP-1 antibody
    • RBAP1 antibody
    • RBBP-3 antibody
    • RBBP3 antibody
    • RBP 3 antibody
    • RBP3 antibody
    • Retinoblastoma-associated protein 1 antibody
    • Retinoblastoma-binding protein 3 antibody
    • Transcription factor E2F1 antibody
    see all

Images

  • Anti-E2F1 antibody [8G9] (ab135251) at 1/500 dilution + Human E2F1 recombinant protein.

    Predicted band size: 47 kDa



    Note: the predicted band size of the full length protein is 47 kDa. The expected band size of the recombinant fragment used here is 42.7 kDa.
  • All lanes : Anti-E2F1 antibody [8G9] (ab135251) at 1/500 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : SK-N-SH cell lysate
    Lane 3 : NIH3T3 cell lysate

    Predicted band size: 47 kDa

  • Flow cytometric analysis of HeLa cells using ab135251 at 1/200 dilution (green) and negative control (red).
  • Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using ab135251 at 1/200 dilution with DAB staining.
  • Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ab135251 at 1/200 dilution with DAB staining.

References

This product has been referenced in:

See all 2 Publications for this product

Customer reviews and Q&As

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