Western blot abreview for Anti-E2F1 antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - other (HeLa cell extracts)
Loading amount
50 µg
Specification
HeLa cell extracts
Gel Running Conditions
Reduced Denaturing (10% SDS page tris glycine)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TNT buffer

Secondary antibody

Name
Non-Abcam antibody was used: biorad anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL
Exposure
30 minute(s) and 0 second(s)
Bands
Specific: 55 kDa Non-specific: 45 kDa

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Submitted May 09 2008

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