Anti-E2F1 antibody (ab245307)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-E2F1 antibody
See all E2F1 primary antibodies -
Description
Rabbit polyclonal to E2F1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-Pmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human E2F1 aa 300-350. The exact sequence is proprietary. (NP_005216.1).
Database link: Q01094 -
Positive control
- IP: HeLa whole cell lysate. IHC-P: Human breast carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab245307 was affinity purified using an epitope specific to E2F1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab245307 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-5 µg/mg of lysate. | |
| IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. -
Sequence similarities
Belongs to the E2F/DP family. -
Post-translational
modificationsPhosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1869 Human
- Omim: 189971 Human
- SwissProt: Q01094 Human
- Unigene: 654393 Human
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Alternative names
- Dmel\CG6376 antibody
- Dmel_CG6376 antibody
- drosE2F1 antibody
see all
Images
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Immunoprecipitation - Anti-E2F1 antibody (ab245307)E2F1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245307 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using an alternative rabbit anti-E2F1 antibody, at 1 µg/ml.
Lane 1: ab245307 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F1 antibody (ab245307)Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for E2F1 using ab245307 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.
Datasheets and documents
References
ab245307 has not yet been referenced specifically in any publications.
