Anti-E2F1 antibody (ab245307)
Key features and details
- Rabbit polyclonal to E2F1
- Suitable for: IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-E2F1 antibody
See all E2F1 primary antibodies -
Description
Rabbit polyclonal to E2F1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human E2F1 aa 300-350. The exact sequence is proprietary. (NP_005216.1).
Database link: Q01094 -
Positive control
- IP: HeLa whole cell lysate. IHC-P: Human breast carcinoma tissue.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab245307 was affinity purified using an epitope specific to E2F1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab245307 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-5 µg/mg of lysate. | |
| IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
-
Function
Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. -
Sequence similarities
Belongs to the E2F/DP family. -
Post-translational
modificationsPhosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 1869 Human
- Omim: 189971 Human
- SwissProt: Q01094 Human
- Unigene: 654393 Human
-
Alternative names
- Dmel\CG6376 antibody
- Dmel_CG6376 antibody
- drosE2F1 antibody
see all
Images
-
Immunoprecipitation - Anti-E2F1 antibody (ab245307)
E2F1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245307 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using an alternative rabbit anti-E2F1 antibody, at 1 µg/ml.
Lane 1: ab245307 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F1 antibody (ab245307)Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for E2F1 using ab245307 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab245307 has not yet been referenced specifically in any publications.
