For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human E2F1 aa 350 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q01094
A trial size is available to purchase for this antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab179445 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 47 kDa.|
|ICC/IF||1/100 - 1/250.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||Use at an assay dependent concentration.|
Western blot analysis on immunoprecipitation pellet from NIH 3T3 cell lysate immunoprecipitated using ab179445 at 1/10 dilution.
Immunohistochemical analysis of breast samples, labeling E2F1 with ab179445.
Immunofluorescent analysis of HepG2 cells labeling E2F1 with ab179445 at 1/100 dilution.
Flow cytometric analysis of permeabilized Hela cells using anti-E2F1 RabMAb (red) (ab179445) or a rabbit IgG (negative) (green)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"