Recombinant Anti-E2F1 antibody [EPR3818(3)] - ChIP Grade (ab179445)
![ChIP - Anti-E2F1 antibody [EPR3818(3)] - ChIP Grade (ab179445)](https://www.abcam.com/ps/products/179/ab179445/Images/ab179445-9-AntiE2F1-antibody-EPR38183ChIP.jpg "ChIP - Anti-E2F1 antibody [EPR3818(3)] - ChIP Grade (ab179445)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3818(3)] to E2F1 - ChIP Grade
- Suitable for: ChIP, WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-E2F1 antibody [EPR3818(3)] - ChIP Grade
See all E2F1 primary antibodies -
Description
Rabbit monoclonal [EPR3818(3)] to E2F1 - ChIP Grade -
Host species
Rabbit -
Tested applications
Suitable for: ChIP, WB, IPmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human E2F1 aa 350 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q01094 -
Positive control
- NIH 3T3, MDA-MB-231, HeLa, HepG2, T47D, Human spleen and fetal muscle lysates; E2F1-DDDDK tag transfected 293T lysate. ChIP: Chromatin prepared from NIH/3T3 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3818(3) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab179445 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ChIP | Use 5 µg for 25 µg of chromatin. | |
| WB | 1/1000 - 1/5000. Predicted molecular weight: 47 kDa. | |
| IP | 1/10 - 1/100. |
Target
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Function
Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. -
Sequence similarities
Belongs to the E2F/DP family. -
Post-translational
modificationsPhosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1869 Human
- Entrez Gene: 13555 Mouse
- Entrez Gene: 399489 Rat
- Omim: 189971 Human
- SwissProt: Q01094 Human
- SwissProt: Q61501 Mouse
- SwissProt: O09139 Rat
- Unigene: 654393 Human
see all -
Alternative names
- Dmel\CG6376 antibody
- Dmel_CG6376 antibody
- drosE2F1 antibody
see all
Images
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ChIP - Anti-E2F1 antibody [EPR3818(3)] - ChIP Grade (ab179445)
Chromatin was prepared from NIH/3T3 cells according to the Abcam X-ChIP protocol. Cells were fixed with 1% formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 5µg of ab179445 (red), and 20µl of protein A/G sepharose beads slurry (10µl of sepharose A beads + 10µl of sepharose G beads). 5μg of rabbit normal IgG was added to the beads control (grey). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
PBS only lot tested -
![Western blot - Anti-E2F1 antibody [EPR3818(3)] - ChIP Grade (ab179445)](https://www.abcam.com/ps/products/179/ab179445/Images/ab179445-393225-anti-e2f1-antibody-epr3818-3-chip-grade-westernblot-hela-human.jpg)
Western blot - Anti-E2F1 antibody [EPR3818(3)] - ChIP Grade (ab179445)All lanes : Anti-E2F1 antibody [EPR3818(3)] - ChIP Grade (ab179445) at 1/1000 dilution
Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 3 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 4 : Human lung lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 9 secondsBlocking/Diluting buffer and concentration 5% NFDM/TBST.
Human normal lung is negative for E2F1 (PMID: 11313916).
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![Immunoprecipitation - Anti-E2F1 antibody [EPR3818(3)] (ab179445)](https://www.abcam.com/ps/products/179/ab179445/Images/ab179445-207437-anti-e2f1-antibody-epr38183-immunoprecipitation.jpg)
Immunoprecipitation - Anti-E2F1 antibody [EPR3818(3)] (ab179445)Western blot analysis on immunoprecipitation pellet from NIH 3T3 cell lysate immunoprecipitated using ab179445 at 1/10 dilution.
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![Western blot - Anti-E2F1 antibody [EPR3818(3)] (ab179445)](https://www.abcam.com/ps/products/179/ab179445/Images/ab179445-207434-anti-e2f1-antibody-epr38183-western-blot.jpg)
Western blot - Anti-E2F1 antibody [EPR3818(3)] (ab179445)All lanes : Anti-E2F1 antibody [EPR3818(3)] - ChIP Grade (ab179445) at 1/1000 dilution
Lane 1 : NIH 3T3 lysate
Lane 2 : HeLa lysate
Lane 3 : HepG2 lysate
Lane 4 : T47D lysate
Lane 5 : Human spleen lysate
Lane 6 : Human fetal muscle lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 47 kDa -
![Anti-E2F1 antibody [EPR3818(3)] - ChIP Grade (ab179445)](https://www.abcam.com/ps/products/179/ab179445/Images/ab179445-12-benefits-of-recombinant-antibodies.png)
Anti-E2F1 antibody [EPR3818(3)] - ChIP Grade (ab179445)
Protocols
Datasheets and documents
References (43)
ab179445 has been referenced in 43 publications.
- Zhou X et al. Long noncoding RNA BSN-AS2 induced by E2F1 promotes spinal osteosarcoma progression by targeting miR-654-3p/SYTL2 axis. Cancer Cell Int 20:133 (2020). PubMed: 32351327
- Xiong Y et al. WFDC2 suppresses prostate cancer metastasis by modulating EGFR signaling inactivation. Cell Death Dis 11:537 (2020). PubMed: 32678075
- Lin Y et al. Exosomes derived from HeLa cells break down vascular integrity by triggering endoplasmic reticulum stress in endothelial cells. J Extracell Vesicles 9:1722385 (2020). PubMed: 32128072
- Meng Q et al. LINC00461/miR-4478/E2F1 feedback loop promotes non-small cell lung cancer cell proliferation and migration. Biosci Rep 40:N/A (2020). PubMed: 31934717
- Zhao X miR-1258 Regulates Cell Proliferation and Cell Cycle to Inhibit the Progression of Breast Cancer by Targeting E2F1. Biomed Res Int 2020:1480819 (2020). PubMed: 32733928
