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    e2f1-antibody-epr38183-chip-grade-ab179445.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Forkhead Box
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RecombinantRabMAb

Recombinant Anti-E2F1 antibody [EPR3818(3)] - ChIP Grade (ab179445)

  • Datasheet
Reviews (1) Submit a question References (43)

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ChIP - Anti-E2F1 antibody [EPR3818(3)] - ChIP Grade (ab179445)
  • Western blot - Anti-E2F1 antibody [EPR3818(3)] - ChIP Grade (ab179445)
  • Immunoprecipitation - Anti-E2F1 antibody [EPR3818(3)] (ab179445)
  • Western blot - Anti-E2F1 antibody [EPR3818(3)] (ab179445)
  • Anti-E2F1 antibody [EPR3818(3)] - ChIP Grade (ab179445)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3818(3)] to E2F1 - ChIP Grade
  • Suitable for: ChIP, WB, IP
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-E2F1 antibody [EPR3818(3)] - ChIP Grade
    See all E2F1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3818(3)] to E2F1 - ChIP Grade
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ChIP, WB, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human E2F1 aa 350 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q01094

  • Positive control

    • NIH 3T3, MDA-MB-231, HeLa, HepG2, T47D, Human spleen and fetal muscle lysates; E2F1-DDDDK tag transfected 293T lysate. ChIP: Chromatin prepared from NIH/3T3 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR3818(3)
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Rb family
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP antibodies

Associated products

  • Alternative Versions

    • Anti-E2F1 antibody [EPR3818(3)] - BSA and Azide free (ab218527)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • T47D whole cell lysate (ab14899)
    • HeLa whole cell lysate (ab150035)
    • HepG2 whole cell lysate (ab166833)
    • HeLa whole cell lysate (ab29545)
    • NIH 3T3 whole cell lysate (ab7179)
  • Recombinant Protein

    • Recombinant Human E2F1 protein (ab82207)
  • Related Products

    • Recombinant Human E2F1 protein (ab82207)

Applications

Our Abpromise guarantee covers the use of ab179445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use 5 µg for 25 µg of chromatin.
WB 1/1000 - 1/5000. Predicted molecular weight: 47 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.
    • Sequence similarities

      Belongs to the E2F/DP family.
    • Post-translational
      modifications

      Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
      Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession Q01094 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1869 Human
      • Entrez Gene: 13555 Mouse
      • Entrez Gene: 399489 Rat
      • Omim: 189971 Human
      • SwissProt: Q01094 Human
      • SwissProt: Q61501 Mouse
      • SwissProt: O09139 Rat
      • Unigene: 654393 Human
      • Unigene: 18036 Mouse
      • Unigene: 72471 Rat
      see all
    • Alternative names

      • Dmel\CG6376 antibody
      • Dmel_CG6376 antibody
      • drosE2F1 antibody
      • E(Sev-CycE)3A antibody
      • E(var)3-93E antibody
      • E2-promoter binding facto antibody
      • E2F 1 antibody
      • E2F transcription factor 1 antibody
      • E2F-1 antibody
      • E2f-PA antibody
      • E2f-PB antibody
      • E2f-PC antibody
      • E2F1 antibody
      • E2f1 E2F transcription factor 1 antibody
      • E2F1_HUMAN antibody
      • Evar(3)164 antibody
      • KIAA4009 antibody
      • l(3)07172 antibody
      • l(3)j3B1 antibody
      • l(3)j3C2 antibody
      • l(3)rM729 antibody
      • mKIAA4009 antibody
      • OTTHUMP00000030661 antibody
      • PBR3 antibody
      • PRB binding protein E2F 1 antibody
      • PRB-binding protein E2F-1 antibody
      • RBAP 1 antibody
      • RBAP-1 antibody
      • RBAP1 antibody
      • RBBP-3 antibody
      • RBBP3 antibody
      • RBP 3 antibody
      • RBP3 antibody
      • Retinoblastoma-associated protein 1 antibody
      • Retinoblastoma-binding protein 3 antibody
      • Transcription factor E2F1 antibody
      see all

    Images

    • ChIP - Anti-E2F1 antibody [EPR3818(3)] - ChIP Grade (ab179445)
      ChIP - Anti-E2F1 antibody [EPR3818(3)] - ChIP Grade (ab179445)

      Chromatin was prepared from NIH/3T3 cells according to the Abcam X-ChIP protocol. Cells were fixed with 1% formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 5µg of ab179445 (red), and 20µl of protein A/G sepharose beads slurry (10µl of sepharose A beads + 10µl of sepharose G beads). 5μg of rabbit normal IgG was added to the beads control (grey). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).

      PBS only lot tested

    • Western blot - Anti-E2F1 antibody [EPR3818(3)] - ChIP Grade (ab179445)
      Western blot - Anti-E2F1 antibody [EPR3818(3)] - ChIP Grade (ab179445)
      All lanes : Anti-E2F1 antibody [EPR3818(3)] - ChIP Grade (ab179445) at 1/1000 dilution

      Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
      Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 3 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysates
      Lane 4 : Human lung lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 47 kDa
      Observed band size: 70 kDa
      why is the actual band size different from the predicted?


      Exposure time: 9 seconds


      Blocking/Diluting buffer and concentration 5% NFDM/TBST.

      Human normal lung is negative for E2F1 (PMID: 11313916).

    • Immunoprecipitation - Anti-E2F1 antibody [EPR3818(3)] (ab179445)
      Immunoprecipitation - Anti-E2F1 antibody [EPR3818(3)] (ab179445)

      Western blot analysis on immunoprecipitation pellet from NIH 3T3 cell lysate immunoprecipitated using ab179445 at 1/10 dilution.

    • Western blot - Anti-E2F1 antibody [EPR3818(3)] (ab179445)
      Western blot - Anti-E2F1 antibody [EPR3818(3)] (ab179445)
      All lanes : Anti-E2F1 antibody [EPR3818(3)] - ChIP Grade (ab179445) at 1/1000 dilution

      Lane 1 : NIH 3T3 lysate
      Lane 2 : HeLa lysate
      Lane 3 : HepG2 lysate
      Lane 4 : T47D lysate
      Lane 5 : Human spleen lysate
      Lane 6 : Human fetal muscle lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 47 kDa

    • Anti-E2F1 antibody [EPR3818(3)] - ChIP Grade (ab179445)
      Anti-E2F1 antibody [EPR3818(3)] - ChIP Grade (ab179445)

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (43)

    Publishing research using ab179445? Please let us know so that we can cite the reference in this datasheet.

    ab179445 has been referenced in 43 publications.

    • Zhou X  et al. Long noncoding RNA BSN-AS2 induced by E2F1 promotes spinal osteosarcoma progression by targeting miR-654-3p/SYTL2 axis. Cancer Cell Int 20:133 (2020). PubMed: 32351327
    • Xiong Y  et al. WFDC2 suppresses prostate cancer metastasis by modulating EGFR signaling inactivation. Cell Death Dis 11:537 (2020). PubMed: 32678075
    • Lin Y  et al. Exosomes derived from HeLa cells break down vascular integrity by triggering endoplasmic reticulum stress in endothelial cells. J Extracell Vesicles 9:1722385 (2020). PubMed: 32128072
    • Meng Q  et al. LINC00461/miR-4478/E2F1 feedback loop promotes non-small cell lung cancer cell proliferation and migration. Biosci Rep 40:N/A (2020). PubMed: 31934717
    • Zhao X miR-1258 Regulates Cell Proliferation and Cell Cycle to Inhibit the Progression of Breast Cancer by Targeting E2F1. Biomed Res Int 2020:1480819 (2020). PubMed: 32733928
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunocytochemistry/ Immunofluorescence abreview for Anti-E2F1 antibody [EPR3818(3)]

    Inconclusive
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Brain)
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    Triton as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.3% · Temperature: rt°C
    Fixative
    OCT
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 12 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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