Recombinant Anti-E2F1 antibody [EPR3818(3)] - ChIP Grade (ab179445)
Rabbit recombinant monoclonal E2F1 antibody [EPR3818(3)]. Validated in WB, IP, IHC, Flow Cyt, ChIP, ICC/IF and tested in Mouse, Rat, Human. Cited in 16 publication(s).
- Datasheet
- References (27)
- Protocols
Overview
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Product name
Anti-E2F1 antibody [EPR3818(3)] - ChIP Grade
See all E2F1 primary antibodies -
Description
Rabbit monoclonal [EPR3818(3)] to E2F1 - ChIP Grade -
Host species
Rabbit -
Tested applications
Suitable for: ChIP, WB, ICC/IF, IP, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human E2F1 aa 350 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q01094 -
Positive control
- NIH 3T3, HeLa, HepG2, T47D, Human spleen and fetal muscle lysates; E2F1-DDDDK tag transfected 293T lysate; HepG2 cells. ChIP: Chromatin prepared from NIH/3T3 cells.
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General notes
A trial size is available to purchase for this antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3818(3) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-E2F1 antibody [EPR3818(3)] (Alexa Fluor® 647) (ab200850)
- Anti-E2F1 antibody [EPR3818(3)] (Alexa Fluor® 488) (ab201243)
- Anti-E2F1 antibody [EPR3818(3)] (Alexa Fluor® 594) (ab208077)
- Anti-E2F1 antibody [EPR3818(3)] (Alexa Fluor® 555) (ab208078)
- Anti-E2F1 antibody [EPR3818(3)] (Phycoerythrin) (ab208871)
- Anti-E2F1 antibody [EPR3818(3)] - BSA and Azide free (ab218527)
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab179445 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ChIP | Use 5 µg for 25 µg of chromatin. | |
| WB | 1/1000 - 1/5000. Predicted molecular weight: 47 kDa. | |
| ICC/IF | 1/100 - 1/250. | |
| IP | 1/10 - 1/100. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IHC-P | Use at an assay dependent concentration. |
Target
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Function
Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. -
Sequence similarities
Belongs to the E2F/DP family. -
Post-translational
modificationsPhosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1869 Human
- Entrez Gene: 13555 Mouse
- Entrez Gene: 399489 Rat
- Omim: 189971 Human
- SwissProt: Q01094 Human
- SwissProt: Q61501 Mouse
- SwissProt: O09139 Rat
- Unigene: 654393 Human
see all -
Alternative names
- Dmel\CG6376 antibody
- Dmel_CG6376 antibody
- drosE2F1 antibody
see all
Images
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![ChIP - Anti-E2F1 antibody [EPR3818(3)] - ChIP Grade (ab179445)](https://www.abcam.com/ps/products/179/ab179445/Images/ab179445-9-AntiE2F1-antibody-EPR38183ChIP.jpg)
ChIP - Anti-E2F1 antibody [EPR3818(3)] - ChIP Grade (ab179445)Chromatin was prepared from NIH/3T3 cells according to the Abcam X-ChIP protocol. Cells were fixed with 1% formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 5µg of ab179445 (red), and 20µl of protein A/G sepharose beads slurry (10µl of sepharose A beads + 10µl of sepharose G beads). 5μg of rabbit normal IgG was added to the beads control (grey). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
PBS only lot tested -
![Western blot - Anti-E2F1 antibody [EPR3818(3)] (ab179445)](https://www.abcam.com/ps/products/179/ab179445/Images/ab179445-207434-anti-e2f1-antibody-epr38183-western-blot.jpg)
Western blot - Anti-E2F1 antibody [EPR3818(3)] (ab179445)All lanes : Anti-E2F1 antibody [EPR3818(3)] - ChIP Grade (ab179445) at 1/1000 dilution
Lane 1 : NIH 3T3 lysate
Lane 2 : HeLa lysate
Lane 3 : HepG2 lysate
Lane 4 : T47D lysate
Lane 5 : Human spleen lysate
Lane 6 : Human fetal muscle lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 47 kDa -
![Immunoprecipitation - Anti-E2F1 antibody [EPR3818(3)] (ab179445)](https://www.abcam.com/ps/products/179/ab179445/Images/ab179445-207437-anti-e2f1-antibody-epr38183-immunoprecipitation.jpg)
Immunoprecipitation - Anti-E2F1 antibody [EPR3818(3)] (ab179445)Western blot analysis on immunoprecipitation pellet from NIH 3T3 cell lysate immunoprecipitated using ab179445 at 1/10 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F1 antibody [EPR3818(3)] - ChIP Grade (ab179445)](https://www.abcam.com/ps/products/179/ab179445/Images/ab179445-278131-anti-e2f1-antibody-epr38183-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F1 antibody [EPR3818(3)] - ChIP Grade (ab179445)Zhussupova A et al., PLoS One, 9, e105285, 2014 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunohistochemical analysis of breast samples, labeling E2F1 with ab179445.
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![Immunocytochemistry/ Immunofluorescence - Anti-E2F1 antibody [EPR3818(3)] (ab179445)](https://www.abcam.com/ps/products/179/ab179445/Images/ab179445-207436-anti-e2f1-antibody-epr38183-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-E2F1 antibody [EPR3818(3)] (ab179445)Immunofluorescent analysis of HepG2 cells labeling E2F1 with ab179445 at 1/100 dilution.
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![Flow Cytometry - Anti-E2F1 antibody [EPR3818(3)] (ab179445)](https://www.abcam.com/ps/products/179/ab179445/Images/ab179445-207962-anti-e2f1-antibody-epr38183-flow-cytometry.jpg)
Flow Cytometry - Anti-E2F1 antibody [EPR3818(3)] (ab179445)Flow cytometric analysis of permeabilized Hela cells using anti-E2F1 RabMAb (red) (ab179445) or a rabbit IgG (negative) (green)
Protocols
Datasheets and documents
References
This product has been referenced in:
- Fang E et al. Valproic acid suppresses Warburg effect and tumor progression in neuroblastoma. Biochem Biophys Res Commun 508:9-16 (2019). Read more (PubMed: 30466785) »
- Ji W et al. Combined Androgen receptor blockade overcomes the resistance of breast cancer cells to palbociclib. Int J Biol Sci 15:522-532 (2019). Read more (PubMed: 30745839) »
