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Epigenetics and Nuclear Signaling Transcription Domain Families Forkhead Box
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Anti-E2F1 (phospho S337) antibody (ab135549)

  • Datasheet
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Western blot - Anti-E2F1 (phospho S337) antibody (ab135549)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F1 (phospho S337) antibody (ab135549)

Key features and details

  • Rabbit polyclonal to E2F1 (phospho S337)
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
Recombinant Human E2F1 protein (ab82207)

View more associated products

Overview

  • Product name

    Anti-E2F1 (phospho S337) antibody
    See all E2F1 primary antibodies
  • Description

    Rabbit polyclonal to E2F1 (phospho S337)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide conjugated to KLH, corresponding to amino acid residues surrounding S337 of Human E2F1.

  • Positive control

    • T47D, HepG2, and A2058 cell lysates; Human breast carcinoma tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab135549 is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2 step procedure with control and phosphorylated peptides. The phospho specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Rb family

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • T47D whole cell lysate (ab14899)
  • Recombinant Protein

    • Recombinant Human E2F1 protein (ab82207)

Applications

Our Abpromise guarantee covers the use of ab135549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 47 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.
  • Sequence similarities

    Belongs to the E2F/DP family.
  • Post-translational
    modifications

    Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
    Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q01094 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1869 Human
    • Omim: 189971 Human
    • SwissProt: Q01094 Human
    • Unigene: 654393 Human
    • Alternative names

      • Dmel\CG6376 antibody
      • Dmel_CG6376 antibody
      • drosE2F1 antibody
      • E(Sev-CycE)3A antibody
      • E(var)3-93E antibody
      • E2-promoter binding facto antibody
      • E2F 1 antibody
      • E2F transcription factor 1 antibody
      • E2F-1 antibody
      • E2f-PA antibody
      • E2f-PB antibody
      • E2f-PC antibody
      • E2F1 antibody
      • E2f1 E2F transcription factor 1 antibody
      • E2F1_HUMAN antibody
      • Evar(3)164 antibody
      • KIAA4009 antibody
      • l(3)07172 antibody
      • l(3)j3B1 antibody
      • l(3)j3C2 antibody
      • l(3)rM729 antibody
      • mKIAA4009 antibody
      • OTTHUMP00000030661 antibody
      • PBR3 antibody
      • PRB binding protein E2F 1 antibody
      • PRB-binding protein E2F-1 antibody
      • RBAP 1 antibody
      • RBAP-1 antibody
      • RBAP1 antibody
      • RBBP-3 antibody
      • RBBP3 antibody
      • RBP 3 antibody
      • RBP3 antibody
      • Retinoblastoma-associated protein 1 antibody
      • Retinoblastoma-binding protein 3 antibody
      • Transcription factor E2F1 antibody
      see all

    Images

    • Western blot - Anti-E2F1 (phospho S337) antibody (ab135549)
      Western blot - Anti-E2F1 (phospho S337) antibody (ab135549)
      All lanes : Anti-E2F1 (phospho S337) antibody (ab135549) at 1/100 dilution

      Lane 1 : T47D cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : A2058 cell lysate

      Lysates/proteins at 12.5 µg per lane.

      Predicted band size: 47 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F1 (phospho S337) antibody (ab135549)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F1 (phospho S337) antibody (ab135549)
      Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human breast carcinoma tissue labelling E2F1 (phospho S337) with ab135549 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and AEC staining.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (2)

    Publishing research using ab135549? Please let us know so that we can cite the reference in this datasheet.

    ab135549 has been referenced in 2 publications.

    • Araki T  et al. EGFR Induces E2F1-Mediated Corticotroph Tumorigenesis. J Endocr Soc 1:127-143 (2017). PubMed: 29264472
    • Araki T  et al. E2F1-mediated human POMC expression in ectopic Cushing's syndrome. Endocr Relat Cancer 23:857-870 (2016). PubMed: 27935805

    Customer reviews and Q&As

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