Key features and details
- Rabbit polyclonal to E2F1 (phospho S337)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-E2F1 (phospho S337) antibody
See all E2F1 primary antibodies
DescriptionRabbit polyclonal to E2F1 (phospho S337)
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic phosphopeptide conjugated to KLH, corresponding to amino acid residues surrounding S337 of Human E2F1.
- T47D, HepG2, and A2058 cell lysates; Human breast carcinoma tissue.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab135549 is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2 step procedure with control and phosphorylated peptides. The phospho specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Our Abpromise guarantee covers the use of ab135549 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/500. Predicted molecular weight: 47 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionTranscription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.
Sequence similaritiesBelongs to the E2F/DP family.
modificationsPhosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B.
- Information by UniProt
- Dmel\CG6376 antibody
- Dmel_CG6376 antibody
- drosE2F1 antibody
All lanes : Anti-E2F1 (phospho S337) antibody (ab135549) at 1/100 dilution
Lane 1 : T47D cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : A2058 cell lysate
Lysates/proteins at 12.5 µg per lane.
Predicted band size: 47 kDa
Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human breast carcinoma tissue labelling E2F1 (phospho S337) with ab135549 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and AEC staining.
ab135549 has been referenced in 2 publications.