Anti-E2F1 (phospho S337) antibody (ab135549)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-E2F1 (phospho S337) antibody
See all E2F1 primary antibodies -
Description
Rabbit polyclonal to E2F1 (phospho S337) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic phosphopeptide conjugated to KLH, corresponding to amino acid residues surrounding S337 of Human E2F1.
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Positive control
- T47D, HepG2, and A2058 cell lysates; Human breast carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab135549 is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2 step procedure with control and phosphorylated peptides. The phospho specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab135549 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/100 - 1/500. Predicted molecular weight: 47 kDa. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. -
Sequence similarities
Belongs to the E2F/DP family. -
Post-translational
modificationsPhosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1869 Human
- Omim: 189971 Human
- SwissProt: Q01094 Human
- Unigene: 654393 Human
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Alternative names
- Dmel\CG6376 antibody
- Dmel_CG6376 antibody
- drosE2F1 antibody
see all
Images
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Western blot - Anti-E2F1 (phospho S337) antibody (ab135549)All lanes : Anti-E2F1 (phospho S337) antibody (ab135549) at 1/100 dilution
Lane 1 : T47D cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : A2058 cell lysate
Lysates/proteins at 12.5 µg per lane.
Predicted band size: 47 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F1 (phospho S337) antibody (ab135549)Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human breast carcinoma tissue labelling E2F1 (phospho S337) with ab135549 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and AEC staining.
Datasheets and documents
References
This product has been referenced in:
- Araki T et al. EGFR Induces E2F1-Mediated Corticotroph Tumorigenesis. J Endocr Soc 1:127-143 (2017). Read more (PubMed: 29264472) »
- Araki T et al. E2F1-mediated human POMC expression in ectopic Cushing's syndrome. Endocr Relat Cancer 23:857-870 (2016). Read more (PubMed: 27935805) »
