The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa.
Use at an assay dependent concentration.
Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.
Belongs to the E2F/DP family.
Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B.
Western blot - Anti-E2F1 (phospho S364) antibody  (ab122888)
All lanes : Anti-E2F1 (phospho S364) antibody  (ab122888) at 1 µg/ml
Lane 1 : Untreated MCF cells immunoprecipitated with a pantropic anti-E2F1 antibody Lane 2 : MCF cells treated with etoposide at 10 µM for 16 h, immunoprecipitated with a pantropic anti-E2F1 antibody Lane 3 : MCF cells treated with etoposide at 10 µM for 16 h