Overview

  • Product name
    Anti-E2F1 (phospho S364) antibody
    See all E2F1 primary antibodies
  • Description
    Rabbit polyclonal to E2F1 (phospho S364)
  • Host species
    Rabbit
  • Specificity
    This antibody is specific for the phosphorylated form of E2F1. Minimal cross-reactivity is seen with non-phosphorylated human E2F1 by peptide ELISA.
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse
  • Immunogen

    Synthetic peptide: SRMGSpLRAPV-C conjugated to KLH, corresponding to amino acids 360-369 of Human E2F 1.

Properties

Applications

Our Abpromise guarantee covers the use of ab5391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/250. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).

Target

  • Function
    Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.
  • Sequence similarities
    Belongs to the E2F/DP family.
  • Post-translational
    modifications
    Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
    Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dmel\CG6376 antibody
    • Dmel_CG6376 antibody
    • drosE2F1 antibody
    • E(Sev-CycE)3A antibody
    • E(var)3-93E antibody
    • E2-promoter binding facto antibody
    • E2F 1 antibody
    • E2F transcription factor 1 antibody
    • E2F-1 antibody
    • E2f-PA antibody
    • E2f-PB antibody
    • E2f-PC antibody
    • E2F1 antibody
    • E2f1 E2F transcription factor 1 antibody
    • E2F1_HUMAN antibody
    • Evar(3)164 antibody
    • KIAA4009 antibody
    • l(3)07172 antibody
    • l(3)j3B1 antibody
    • l(3)j3C2 antibody
    • l(3)rM729 antibody
    • mKIAA4009 antibody
    • OTTHUMP00000030661 antibody
    • PBR3 antibody
    • PRB binding protein E2F 1 antibody
    • PRB-binding protein E2F-1 antibody
    • RBAP 1 antibody
    • RBAP-1 antibody
    • RBAP1 antibody
    • RBBP-3 antibody
    • RBBP3 antibody
    • RBP 3 antibody
    • RBP3 antibody
    • Retinoblastoma-associated protein 1 antibody
    • Retinoblastoma-binding protein 3 antibody
    • Transcription factor E2F1 antibody
    see all

Images

  • All lanes : Anti-E2F1 (phospho S364) antibody (ab5391) at 1/250 dilution

    Lane 1 : CRE (mouse) cells transfected with human E2F1
    Lane 2 : CRE (mouse) cells transfected with human E2F1 with doxorubicin
    Lane 3 : NiH3T3 (mouse) cell lysate
    Lane 4 : NiH3T3 (mouse) cell lysate with doxorubicin
    Lane 5 : MDA-231 (human breast cancer) cell lysate
    Lane 6 : MDA-231 (human breast cancer) cell lysate with doxorubicin

    Secondary
    All lanes : HRP conjugated Goat-anti-Rabbit IgG MX10

    Predicted band size: 47 kDa



    Lysates were prepared from untreated cells and cells treated with 2µM Doxorubicin used as a DNA damaging agent. The arrow indicates E2F1.

     Phosphorylated E2F1 was only expressed in the treated cells. The blot clearly shows that ab5391 only detected phosphorylated E2F1 in the human cell lines.

  • ab5391 at 10 μg/ml staining human breast carcinoma.

References

This product has been referenced in:
  • Laine A  et al. Senescence sensitivity of breast cancer cells is defined by positive feedback loop between CIP2A and E2F1. Cancer Discov 3:182-97 (2013). Human . Read more (PubMed: 23306062) »
  • de Olano N  et al. The p38 MAPK-MK2 Axis Regulates E2F1 and FOXM1 Expression after Epirubicin Treatment. Mol Cancer Res 10:1189-202 (2012). Human . Read more (PubMed: 22802261) »
See all 3 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HT29 tumour cell line)
Loading amount
50 µg
Specification
HT29 tumour cell line
Treatment
50um etoposide for 6 hr
Gel Running Conditions
Reduced Denaturing (10% tris glycine)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Mr. Paul Eve

Verified customer

Submitted Apr 25 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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