Key features and details
- Rabbit polyclonal to E2F2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-E2F2 antibody
See all E2F2 primary antibodies
DescriptionRabbit polyclonal to E2F2
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
- WB: HEK-293T whole cell lysate. IHC-P: Human kidney, brain and placenta tissues.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab235837 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 48 kDa.|
FunctionTranscription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F-2 binds specifically to RB1 protein, in a cell-cycle dependent manner.
Tissue specificityHighest level of expression is found in placenta, low levels are found in lung. Found as well in many immortalized cell lines derived from tumor samples.
Sequence similaritiesBelongs to the E2F/DP family.
modificationsPhosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
- Information by UniProt
- dE2F2 antibody
- E2F transcription factor 2 antibody
- E2F-2 antibody
Anti-E2F2 antibody (ab235837) at 1/500 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 48 kDa
Paraffin-embedded human kidney tissue stained for E2F2 using ab235837 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human brain tissue stained for E2F2 using ab235837 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human placenta tissue stained for E2F2 using ab235837 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235837 has not yet been referenced specifically in any publications.