Overview

  • Product name

    Anti-E2F2 antibody [EPR8622]
    See all E2F2 primary antibodies
  • Description

    Rabbit monoclonal [EPR8622] to E2F2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human E2F2 aa 300-400. The exact sequence is proprietary.

  • Positive control

    • K562, 293T, HepG2, and Ramos cell lysates. Ramos cells.
  • General notes

    A trial size is available to purchase for this antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab138515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 47 kDa.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F-2 binds specifically to RB1 protein, in a cell-cycle dependent manner.
    • Tissue specificity

      Highest level of expression is found in placenta, low levels are found in lung. Found as well in many immortalized cell lines derived from tumor samples.
    • Sequence similarities

      Belongs to the E2F/DP family.
    • Post-translational
      modifications

      Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • dE2F2 antibody
      • E2F transcription factor 2 antibody
      • E2F-2 antibody
      • E2F2 antibody
      • E2F2_HUMAN antibody
      • Transcription factor E2F2 antibody
      see all

    Images

    • All lanes : Anti-E2F2 antibody [EPR8622] (ab138515) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : Ramos cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 47 kDa

    • Flow Cytometrical analysis of permeabilized Ramos cells with ab138515 antibody at a dilution of 1/100 (red) or a rabbit IgG negative (green).

    References

    This product has been referenced in:

    • Wei D  et al. Transcriptional Regulation by CpG Sites Methylation in the Core Promoter Region of the Bovine SIX1 Gene: Roles of Histone H4 and E2F2. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 29337851) »
    • Béguelin W  et al. EZH2 enables germinal centre formation through epigenetic silencing of CDKN1A and an Rb-E2F1 feedback loop. Nat Commun 8:877 (2017). Read more (PubMed: 29026085) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab138515.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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