Overview

  • Product name

    Anti-E2F2 antibody - N-terminal
    See all E2F2 primary antibodies
  • Description

    Rabbit polyclonal to E2F2 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human E2F2 (N terminal). The exact sequence is proprietary. Conjugated to a protein carrier.
    Database link: Q14209

  • Positive control

    • WB: K562 and HEK-293T whole cell and nuclear extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab227599 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 47 kDa.

Target

  • Function

    Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F-2 binds specifically to RB1 protein, in a cell-cycle dependent manner.
  • Tissue specificity

    Highest level of expression is found in placenta, low levels are found in lung. Found as well in many immortalized cell lines derived from tumor samples.
  • Sequence similarities

    Belongs to the E2F/DP family.
  • Post-translational
    modifications

    Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • dE2F2 antibody
    • E2F transcription factor 2 antibody
    • E2F-2 antibody
    • E2F2 antibody
    • E2F2_HUMAN antibody
    • Transcription factor E2F2 antibody
    see all

Images

  • All lanes : Anti-E2F2 antibody - N-terminal (ab227599) at 1/500 dilution

    Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell extract
    Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) nuclear extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 47 kDa



    10% SDS-PAGE gel.

  • All lanes : Anti-E2F2 antibody - N-terminal (ab227599) at 1/500 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) nuclear extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 47 kDa



    10% SDS-PAGE gel.

References

ab227599 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227599.
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