Overview

  • Product name
  • Description
    Rabbit polyclonal to E2F3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Mouse E2F3.

    Read Abcam's proprietary immunogen policy (Peptide available as ab99875.)

  • Positive control
    • This antibody gave a positive signal in Brain (Mouse) Tissue Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab74180 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).

Target

  • Function
    Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-3 binds specifically to RB1 protein, in a cell-cycle dependent manner.
  • Sequence similarities
    Belongs to the E2F/DP family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686C18211 antibody
    • E2F 3 antibody
    • E2F transcription factor 3 antibody
    • E2F-3 antibody
    • E2F3 antibody
    • E2F3_HUMAN antibody
    • KIAA0075 antibody
    • MGC104598 antibody
    • Transcription factor E2F 3 antibody
    • Transcription factor E2F3 antibody
    see all

Images

  • Anti-E2F3 antibody (ab74180) at 1 µg/ml + Brain (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 49 kDa
    Observed band size: 49 kDa


    Exposure time: 18 minutes

References

This product has been referenced in:
  • Prystowsky M  et al. Inhibition of Plk1 and Cyclin B1 expression results in panobinostat-induced G2 delay and mitotic defects. Sci Rep 3:2640 (2013). ChIP . Read more (PubMed: 24026482) »
  • Vuong L  et al. Pattern of expression of p53, its family members, and regulators during early ocular development and in the post-mitotic retina. Invest Ophthalmol Vis Sci 53:4821-31 (2012). Read more (PubMed: 22714890) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for your reply and for keeping me updated.

I'm glad to hear that the E2F4 antibody seems to work, and that the p107 antibody is nowshowing the specific band. I ran a BLAST of the p107 immunogen sequence to see if there are other similar proteins that it might be cross-reacting with, but this came up pretty clean. I couldn't find much in the literature either, but a strong band around 50 kDa is also seen with other p107 antibodies (such as ab90507). At this time we haven't characterized the band, so I apologize but I'm not sure what this is.

If you need anything else from me, please don't hesitate to ask.

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Question
Answer

Thank you for your calls earlier this month and for your patience.

Have the replacement vials of ab53060 and ab2451 provided any new results?

I've reviewed the protocols used to test these antibodies, and I didn't notice anything particularly unusual. The only difference was thatBSA was used as the blocking agent for the E2F antibodytestings, while the p107 antibody was tested on a membraned blocked with Blotto (milk based). Some antibodieswork better with a certain blocking agent,though I can't be sure that thisis a cause of the absent bands in this case.

The lab that tested ab2451 did report seeing a band between 55 and 60 kDa in their samples, however this was in addition to a strong specific band at the expected molecular weight. This extra band is believed to be a cleavage fragment, but no further characterization has been done on the band.

Please let me know if there has been any improvement with the results using the replacement antibodies, and if you would like to try a replacement for the E2F3 antibody I will be happy to send one.

I look forward to hearing from you. Let me know if you have any questions or if there is anything else that we can do for you.

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Question
Answer

Thank you for your call today and for letting us know about the trouble with these antibodies. I apologize for the inconvenience.

As we discussed, please let me know if you would like replacements for anything of these antibodies or if you might prefer credits or refunds, and I will be happy to arrange this for you. I will also be in touch with the testing labs to see if there are any specific protocol suggestions when using these antibodies.

I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you, and I'll be happy to help.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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