Overview

  • Product name
    Anti-E2F3 antibody - C-terminal
    See all E2F3 primary antibodies
  • Description
    Goat polyclonal to E2F3 - C-terminal
  • Host species
    Goat
  • Specificity
    ab181476 detects an ~60kDa and 48kDa band in Mouse fetal Lung lysates. The 60kDa band was also observed in nuclear lysates of cell line NIH3T3 and this band corresponds with results from different antibodies from other commercial sources.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide corresponding to Mouse E2F3 aa 444-457 (C terminal). (NP_034223.1).
    Sequence:

    DLEKLPLVEDFMCS


    Database link: O35261

  • Positive control
    • Mouse fetal lung lysate; NIH3T3 nuclear lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 99% Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab181476 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 49 kDa.

Target

  • Function
    Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-3 binds specifically to RB1 protein, in a cell-cycle dependent manner.
  • Sequence similarities
    Belongs to the E2F/DP family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686C18211 antibody
    • E2F 3 antibody
    • E2F transcription factor 3 antibody
    • E2F-3 antibody
    • E2F3 antibody
    • E2F3_HUMAN antibody
    • KIAA0075 antibody
    • MGC104598 antibody
    • Transcription factor E2F 3 antibody
    • Transcription factor E2F3 antibody
    see all

Images

  • All lanes : Anti-E2F3 antibody - C-terminal (ab181476) at 1 µg/ml

    Lane 1 : Mouse fetal lung lysate in RIPA buffer
    Lane 2 : Mouse fetal lung lysate in RIPA buffer with immunizing peptide

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 49 kDa



    Primary incubation was 1 hour.

References

ab181476 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181476.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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