Key features and details
- Rabbit polyclonal to E2F4
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab234997 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/5000. Predicted molecular weight: 44 kDa.|
FunctionTranscription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.
Tissue specificityFound in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Sequence similaritiesBelongs to the E2F/DP family.
Developmental stagePresent in the growth-arrested state, its abundance does not change significantly as cells move into and through the cell cycle.
modificationsDifferentially phosphorylated in vivo.
- Information by UniProt
- E2F 4 antibody
- E2F transcription factor 4 antibody
- E2F transcription factor 4 p107/p130 binding antibody
Paraffin-embedded human lung tissue stained for E2F4 using ab234997 at 1/100 dilution in immunohistochemical analysis.
Anti-E2F4 antibody (ab234997) at 1/500 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Polyclonal Goat anti-rabbit IgG at 1/50000 dilution
Predicted band size: 44 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab234997 has been referenced in 1 publication.
- Hong J et al. Competitive regulation by transcription factors and DNA methylation in the bovine SIRT5 promoter: Roles of E2F4 and KLF6. Gene 684:39-46 (2019). PubMed: 30359737