Product nameAnti-E2F4 antibody
See all E2F4 primary antibodies
DescriptionRabbit polyclonal to E2F4
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human E2F4 aa 363-413. The exact sequence is proprietary. NP_001941.2
Database link: Q16254
- IHC-P: Human prostate carcinoma tissue; IP: HeLa whole cell lysate; WB: HeLa and Hek-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 44 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
FunctionTranscription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.
Tissue specificityFound in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Sequence similaritiesBelongs to the E2F/DP family.
Developmental stagePresent in the growth-arrested state, its abundance does not change significantly as cells move into and through the cell cycle.
modificationsDifferentially phosphorylated in vivo.
- Information by UniProt
- E2F 4 antibody
- E2F transcription factor 4 antibody
- E2F transcription factor 4 p107/p130 binding antibody
All lanes : Anti-E2F4 antibody (ab264276) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : Hek-293T whole cell lysate at 50 µg
Predicted band size: 44 kDa
Detected with chemilimuniscence with exposure time of 30 seconds.
Immunohistochemical analysis of FFPE human prostate carcinoma tissue section labelling E2F4 with ab264276 at 1/1000 dilution. Detection: DAB.
E2F4 was immunoprecipitated from HEK-293T whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264276 at 6 μg per reaction. Western blot was performed on the immunoprecipitates using ab264276 at 1 μg/ml.
Lane 1: ab264276 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Exposure time:10 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264276 has not yet been referenced specifically in any publications.