Overview

  • Product name

    Anti-E2F4 antibody [EPR8259] - BSA and Azide free
    See all E2F4 primary antibodies
  • Description

    Rabbit monoclonal [EPR8259] to E2F4 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human E2F4 aa 250-350. The exact sequence is proprietary.
    Database link: Q16254

  • General notes

    Ab240118 is the carrier-free version of ab150360. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240118 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 44 kDa).
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.
    • Tissue specificity

      Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
    • Sequence similarities

      Belongs to the E2F/DP family.
    • Developmental stage

      Present in the growth-arrested state, its abundance does not change significantly as cells move into and through the cell cycle.
    • Post-translational
      modifications

      Differentially phosphorylated in vivo.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • E2F 4 antibody
      • E2F transcription factor 4 antibody
      • E2F transcription factor 4 p107/p130 binding antibody
      • E2F-4 antibody
      • E2F4 antibody
      • E2F4_HUMAN antibody
      • p107/p130 binding protein antibody
      • Transcription factor E2F 4 antibody
      • Transcription factor E2F4 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling E2F4 with ab150360 antibody at a dilution of 1/250.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150360).

    • Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue labelling E2F4 with ab150360 antibody at a dilution of 1/250.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150360).

    • Immunofluorescent analysis of HeLa cells labelling E2F4 with ab150360 at 1/250 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150360).

    • Immunohistochemical analysis of paraffin embedded Human Thyroid gland papillary carcinoma tissue using ab150360 showing +ve staining.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150360).

    • Immunohistochemical analysis of paraffin embedded Normal Human Colon tissue using ab150360 showing +ve staining.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150360).

    • Immunohistochemical analysis of paraffin embedded Human Colonic adenocarcinoma tissue using ab150360 showing +ve staining.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150360).

    • Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab150360 showing +ve staining.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150360).

    References

    ab240118 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab240118.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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