Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8259] to E2F4 - BSA and Azide free
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Human
Product nameAnti-E2F4 antibody [EPR8259] - BSA and Azide free
See all E2F4 primary antibodies
DescriptionRabbit monoclonal [EPR8259] to E2F4 - BSA and Azide free
Tested applicationsSuitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP
Species reactivityReacts with: Human
Synthetic peptide within Human E2F4 aa 250-350. The exact sequence is proprietary.
Database link: Q16254
- WB: K562, Raji, HeLa, and Jurkat (ab7899) cell lysates. IHC-P: Human kidney and lung adenocarcinoma tissues.
ab240118 is the carrier-free version of ab150360. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab240118 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
KO cell lines
KO cell lysates
Our Abpromise guarantee covers the use of ab240118 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 44 kDa).|
FunctionTranscription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.
Tissue specificityFound in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Sequence similaritiesBelongs to the E2F/DP family.
Developmental stagePresent in the growth-arrested state, its abundance does not change significantly as cells move into and through the cell cycle.
modificationsDifferentially phosphorylated in vivo.
- Information by UniProt
- E2F 4 antibody
- E2F transcription factor 4 antibody
- E2F transcription factor 4 p107/p130 binding antibody
All lanes : Anti-E2F4 antibody [EPR8259] (ab150360) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : E2F4 knockout HEK293T cell lysate
Lane 3 : K-562 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 44 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
This data was developed using the same antibody clone in a different buffer formulation (ab150360).
ab150360 Anti-E2F4 antibody [EPR8259] was shown to specifically react with E2F4 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266119 (knockout cell lysate ab257932) was used. Wild-type and E2F4 knockout samples were subjected to SDS-PAGE. ab150360 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab240118 has not yet been referenced specifically in any publications.