Overview

  • Product name

  • Description

    Rabbit polyclonal to E2F5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment within Human E2F5 (internal sequence). The exact sequence is proprietary.
    Database link: Q15329

  • Positive control

    • WB: HA-human E2F5-transfected HEK-293T cell extract.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227587 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 38 kDa.

Target

  • Function

    Transcriptional activator that binds to E2F sites, these sites are present in the promoter of many genes whose products are involved in cell proliferation. May mediate growth factor-initiated signal transduction. It is likely involved in the early responses of resting cells to growth factor stimulation.
  • Sequence similarities

    Belongs to the E2F/DP family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • E2F 5 antibody
    • E2F transcription factor 5 antibody
    • E2F transcription factor 5 p130 binding antibody
    • E2F-5 antibody
    • E2f5 antibody
    • E2F5_HUMAN antibody
    • p130 binding antibody
    • Transcription factor E2F5 antibody
    see all

Images

  • All lanes : Anti-E2F5 antibody (ab227587) at 1/10000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with HA-human E2F5, whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 38 kDa



    10% SDS-PAGE gel.

References

ab227587 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227587.
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