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The new lot of anti-p107 is still showing the lower band that is very strong, but we now see the band of interest at the right size as well. The E2F4 seems to work too.
Asked on Mar 29 2012
Thank you for your reply and for keeping me updated.
I'm glad to hear that the E2F4 antibody seems to work, and that the p107 antibody is nowshowing the specific band. I ran a BLAST of the p107 immunogen sequence to see if there are other similar proteins that it might be cross-reacting with, but this came up pretty clean. I couldn't find much in the literature either, but a strong band around 50 kDa is also seen with other p107 antibodies (such as ab90507). At this time we haven't characterized the band, so I apologize but I'm not sure what this is.
If you need anything else from me, please don't hesitate to ask.
Answered on Mar 29 2012