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Synthetic peptide within Human E2F5 aa 72-102 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q62814
Our Abpromise guarantee covers the use of ab176017 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000. Predicted molecular weight: 38 kDa.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human hepatocarcinoma tissue labeling E2F5 with ab176017 at 1/50 dilution, followed by peroxidase conjugation to the secondary antibody and DAB staining.
Flow cytometric analysis of ZR-75-1 cells labeling E2F5 with ab176017 at 1/10 dilution (bottom histogram), compared to a negative control cell (top histogram). FITC-conjugated Goat anti-Rabbit secondary antibodies were used for the analysis.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"