Key features and details
- Rabbit polyclonal to E2F6
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-E2F6 antibody
See all E2F6 primary antibodies
DescriptionRabbit polyclonal to E2F6
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to a region within amino acids 59-281 of E2F6 (UniProt ID: O75461).
- A549 whole cell lysate; H441 xenograft tissue; A549 cells
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab152151 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 32 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
FunctionInhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F-6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase.
Tissue specificityExpressed in all tissues examined. Highest levels in placenta, skeletal muscle, heart, ovary, kidney, small intestine and spleen.
Sequence similaritiesBelongs to the E2F/DP family.
- Information by UniProt
- E2F 6 antibody
- E2F binding site modulating activity protein antibody
- E2F transcription factor 6 antibody
Anti-E2F6 antibody (ab152151) at 1/1000 dilution + A549 whole cell lysate at 30 µg
Predicted band size: 32 kDa
12% SDS PAGE
Immunohistochemical analysis of paraffin embedded H441 xenograft tissue, labeling E2F6 using ab152151 at 1/500 dilution.
Immunofluorescence analysis of paraformaldehyde fixed A549 cells, labeling E2F6 using ab152151 at 1/500 dilution. Lower image costained with Hoerchst 33342.
ab152151 has been referenced in 2 publications.
- Lu Z et al. MicroRNA-424/E2F6 feedback loop modulates cell invasion, migration and EMT in endometrial carcinoma. Oncotarget 8:114281-114291 (2017). PubMed: 29371986
- Korourian A et al. MicroRNA-31 inhibits RhoA-mediated tumor invasion and chemotherapy resistance in MKN-45 gastric adenocarcinoma cells. Exp Biol Med (Maywood) 242:1842-1847 (2017). PubMed: 28836853