Recombinant Anti-E2F6 antibody [EPR11201] (ab155978)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11201] to E2F6
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-E2F6 antibody [EPR11201]
See all E2F6 primary antibodies -
Description
Rabbit monoclonal [EPR11201] to E2F6 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human E2F6.
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Positive control
- Jurkat, K562, HeLa, A549 and HepG2 cell lysates; HeLa and K562 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11201 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155978 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 32 kDa.
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ICC/IF |
1/100 - 1/250.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 32 kDa. |
ICC/IF
1/100 - 1/250. |
Target
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Function
Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F-6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase. -
Tissue specificity
Expressed in all tissues examined. Highest levels in placenta, skeletal muscle, heart, ovary, kidney, small intestine and spleen. -
Sequence similarities
Belongs to the E2F/DP family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1876 Human
- Omim: 602944 Human
- SwissProt: O75461 Human
- Unigene: 603093 Human
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Alternative names
- E2F 6 antibody
- E2F binding site modulating activity protein antibody
- E2F transcription factor 6 antibody
see all
Images
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All lanes : Anti-E2F6 antibody [EPR11201] (ab155978) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : A549 cell lysate
Lane 5 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 32 kDa -
Immunofluorescent analysis of HeLa cells, labeling E2F6 with ab155978 at 1/100 dilution.
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Intracellular flow cytometric analysis of permeabilized K562 cells labeling E2F6 (red), with ab155978 at 1/10 dilution, or a Rabbit IgG (negative) (green)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab155978 has been referenced in 2 publications.
- Pennycook BR et al. E2F-dependent transcription determines replication capacity and S phase length. Nat Commun 11:3503 (2020). PubMed: 32665547
- Zheng Y et al. Silencing of Long Non-Coding RNA LINC00607 Prevents Tumor Proliferation of Osteosarcoma by Acting as a Sponge of miR-607 to Downregulate E2F6. Front Oncol 10:584452 (2020). PubMed: 33585204