Recombinant
RabMAb

Anti-E2F6 antibody [EPR11201] (ab155978)

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Overview

  • Product name
    Anti-E2F6 antibody [EPR11201]
    See all E2F6 primary antibodies
  • Description
    Rabbit monoclonal [EPR11201] to E2F6
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant protein fragment, corresponding to residues in Human E2F6 (Uniprot: O75461).

  • Positive control
    • Jurkat, K562, HeLa, A549 and HepG2 cell lysates; HeLa and K562 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab155978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 32 kDa.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Function
      Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F-6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase.
    • Tissue specificity
      Expressed in all tissues examined. Highest levels in placenta, skeletal muscle, heart, ovary, kidney, small intestine and spleen.
    • Sequence similarities
      Belongs to the E2F/DP family.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
      • Alternative names
        • E2F 6 antibody
        • E2F binding site modulating activity protein antibody
        • E2F transcription factor 6 antibody
        • E2F transcription factor 6 isoform 1 antibody
        • E2F-6 antibody
        • E2F6 antibody
        • E2F6_HUMAN antibody
        • EMA antibody
        • MGC111545 antibody
        • Transcription factor E2F6 antibody
        see all

      Images

      • Western blot - Anti-E2F6 antibody [EPR11201] (ab155978)
        Western blot - Anti-E2F6 antibody [EPR11201] (ab155978)
        All lanes : Anti-E2F6 antibody [EPR11201] (ab155978) at 1/1000 dilution

        Lane 1 : Jurkat cell lysate
        Lane 2 : K562 cell lysate
        Lane 3 : HeLa cell lysate
        Lane 4 : A549 cell lysate
        Lane 5 : HepG2 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 32 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-E2F6 antibody [EPR11201] (ab155978)
        Immunocytochemistry/ Immunofluorescence - Anti-E2F6 antibody [EPR11201] (ab155978)
        Immunofluorescent analysis of HeLa cells, labeling E2F6 with ab155978 at 1/100 dilution.
      • Flow Cytometry - Anti-E2F6 antibody [EPR11201] (ab155978)
        Flow Cytometry - Anti-E2F6 antibody [EPR11201] (ab155978)
        Flow cytometric analysis of permeabilized K562 cells labeling E2F6 (red), with ab155978 at 1/10 dilution, or a Rabbit IgG (negative) (green)

      References

      ab155978 has not yet been referenced specifically in any publications.

      Publishing research using ab155978? Please let us know so that we can cite the reference in this datasheet.

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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