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    e2f6-antibody-epr11201-ab155978.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Forkhead Box
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RecombinantRabMAb

Recombinant Anti-E2F6 antibody [EPR11201] (ab155978)

  • Datasheet
  • SDS
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Western blot - Anti-E2F6 antibody [EPR11201] (ab155978)
  • Immunocytochemistry/ Immunofluorescence - Anti-E2F6 antibody [EPR11201] (ab155978)
  • Flow Cytometry - Anti-E2F6 antibody [EPR11201] (ab155978)
  • Anti-E2F6 antibody [EPR11201] (ab155978)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11201] to E2F6
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human

You may also be interested in

Protein
Product image
Recombinant Human E2F6 protein (ab114573)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-E2F6 antibody [EPR11201]
    See all E2F6 primary antibodies
  • Description

    Rabbit monoclonal [EPR11201] to E2F6
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human E2F6.

  • Positive control

    • Jurkat, K562, HeLa, A549 and HepG2 cell lysates; HeLa and K562 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11201
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors

Associated products

  • Alternative Versions

    • Anti-E2F6 antibody [EPR11201] - BSA and Azide free (ab249226)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Recombinant Human E2F6 protein (ab114573)
    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab7899)
    • A549 whole cell lysate (ab7910)
    • K-562 whole cell lysate (ab7911)
  • Recombinant Protein

    • Recombinant Human E2F6 protein (ab114573)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Mouse
Human
WB
Human
Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 32 kDa.
ICC/IF
1/100 - 1/250.
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 32 kDa.
ICC/IF
1/100 - 1/250.
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F-6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase.
    • Tissue specificity

      Expressed in all tissues examined. Highest levels in placenta, skeletal muscle, heart, ovary, kidney, small intestine and spleen.
    • Sequence similarities

      Belongs to the E2F/DP family.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession O75461 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1876 Human
      • Omim: 602944 Human
      • SwissProt: O75461 Human
      • Unigene: 603093 Human
      • Alternative names

        • E2F 6 antibody
        • E2F binding site modulating activity protein antibody
        • E2F transcription factor 6 antibody
        • E2F transcription factor 6 isoform 1 antibody
        • E2F-6 antibody
        • E2F6 antibody
        • E2F6_HUMAN antibody
        • EMA antibody
        • MGC111545 antibody
        • Transcription factor E2F6 antibody
        see all

      Images

      • Western blot - Anti-E2F6 antibody [EPR11201] (ab155978)
        Western blot - Anti-E2F6 antibody [EPR11201] (ab155978)
        All lanes : Anti-E2F6 antibody [EPR11201] (ab155978) at 1/1000 dilution

        Lane 1 : Jurkat cell lysate
        Lane 2 : K562 cell lysate
        Lane 3 : HeLa cell lysate
        Lane 4 : A549 cell lysate
        Lane 5 : HepG2 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 32 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-E2F6 antibody [EPR11201] (ab155978)
        Immunocytochemistry/ Immunofluorescence - Anti-E2F6 antibody [EPR11201] (ab155978)
        Immunofluorescent analysis of HeLa cells, labeling E2F6 with ab155978 at 1/100 dilution.
      • Flow Cytometry - Anti-E2F6 antibody [EPR11201] (ab155978)
        Flow Cytometry - Anti-E2F6 antibody [EPR11201] (ab155978)
        Flow cytometric analysis of permeabilized K562 cells labeling E2F6 (red), with ab155978 at 1/10 dilution, or a Rabbit IgG (negative) (green)
      • Anti-E2F6 antibody [EPR11201] (ab155978)
        Anti-E2F6 antibody [EPR11201] (ab155978)

      Protocols

      • Flow cytometry protocols
      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (1)

      Publishing research using ab155978? Please let us know so that we can cite the reference in this datasheet.

      ab155978 has been referenced in 1 publication.

      • Pennycook BR  et al. E2F-dependent transcription determines replication capacity and S phase length. Nat Commun 11:3503 (2020). PubMed: 32665547

      Customer reviews and Q&As

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      There are currently no Customer reviews or Questions for ab155978.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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