Recombinant Anti-E2F6 antibody [EPR11201] (ab155978)
![Western blot - Anti-E2F6 antibody [EPR11201] (ab155978)](https://www.abcam.com/ps/products/155/ab155978/Images/ab155978-163288-anti-e2f6-antibody-epr11201-western-blot.jpg "Western blot - Anti-E2F6 antibody [EPR11201] (ab155978)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11201] to E2F6
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-E2F6 antibody [EPR11201]
See all E2F6 primary antibodies -
Description
Rabbit monoclonal [EPR11201] to E2F6 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human E2F6.
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Positive control
- Jurkat, K562, HeLa, A549 and HepG2 cell lysates; HeLa and K562 cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11201 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155978 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
|
| ICC/IF |
Human
|
| IHC-P |
Mouse
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 32 kDa.
|
|
| ICC/IF |
1/100 - 1/250.
|
|
| Flow Cyt |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 32 kDa. |
|
ICC/IF
1/100 - 1/250. |
|
Flow Cyt
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F-6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase. -
Tissue specificity
Expressed in all tissues examined. Highest levels in placenta, skeletal muscle, heart, ovary, kidney, small intestine and spleen. -
Sequence similarities
Belongs to the E2F/DP family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1876 Human
- Omim: 602944 Human
- SwissProt: O75461 Human
- Unigene: 603093 Human
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Alternative names
- E2F 6 antibody
- E2F binding site modulating activity protein antibody
- E2F transcription factor 6 antibody
see all
Images
-
Western blot - Anti-E2F6 antibody [EPR11201] (ab155978)All lanes : Anti-E2F6 antibody [EPR11201] (ab155978) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : A549 cell lysate
Lane 5 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 32 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-E2F6 antibody [EPR11201] (ab155978)](https://www.abcam.com/ps/products/155/ab155978/Images/ab155978-163287-anti-e2f6-antibody-epr11201-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-E2F6 antibody [EPR11201] (ab155978)Immunofluorescent analysis of HeLa cells, labeling E2F6 with ab155978 at 1/100 dilution. -
![Flow Cytometry - Anti-E2F6 antibody [EPR11201] (ab155978)](https://www.abcam.com/ps/products/155/ab155978/Images/ab155978-163286-anti-e2f6-antibody-epr11201-flow-cytometry.jpg)
Flow Cytometry - Anti-E2F6 antibody [EPR11201] (ab155978)Flow cytometric analysis of permeabilized K562 cells labeling E2F6 (red), with ab155978 at 1/10 dilution, or a Rabbit IgG (negative) (green) -
![Anti-E2F6 antibody [EPR11201] (ab155978)](https://www.abcam.com/ps/products/155/ab155978/Images/ab155978-4-benefits-of-recombinant-antibodies.png)
Anti-E2F6 antibody [EPR11201] (ab155978)
Protocols
References (1)
ab155978 has been referenced in 1 publication.
- Pennycook BR et al. E2F-dependent transcription determines replication capacity and S phase length. Nat Commun 11:3503 (2020). PubMed: 32665547
