Anti-E2F6 antibody [TFE61] (ab11952)
- Datasheet
- References (4)
- Protocols
Overview
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Product nameAnti-E2F6 antibody [TFE61]
See all E2F6 primary antibodies -
DescriptionMouse monoclonal [TFE61] to E2F6
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Host speciesMouse
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Tested applicationsSuitable for: IP, ICC/IF, WBmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant full length protein (Human)
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Positive control
- Hela cells or K-562 nuclear extract.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferConstituent: PBS
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Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberTFE61
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MyelomaSp2/0-Ag14
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab11952 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | ||
| ICC/IF | ||
| WB |
Application notes
WB: 1/500 - 1/1000. Detects a band of approximately 38 kDa from K-562 nuclear extract(predicted molecular weight: 32 kDa). For optimal results, primary antibody incubations should be performed at 37°C
ICC/IF: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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FunctionInhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F-6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase.
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Tissue specificityExpressed in all tissues examined. Highest levels in placenta, skeletal muscle, heart, ovary, kidney, small intestine and spleen.
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Sequence similaritiesBelongs to the E2F/DP family.
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Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 1876 Human
- Omim: 602944 Human
- SwissProt: O75461 Human
- Unigene: 603093 Human
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Alternative names
- E2F 6 antibody
- E2F binding site modulating activity protein antibody
- E2F transcription factor 6 antibody
see all
Images
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![Western blot - Anti-E2F6 antibody [TFE61] (ab11952)](https://www.abcam.com/ps/products/11/ab11952/Images/ACFAF8.jpg)
Western blot - Anti-E2F6 antibody [TFE61] (ab11952)Detection of E2F6 by Western blot analysis. E2F6 is detected in nuclear extracts derived from K-562 cells using a 1/1000 dilution of ab11952.
Detection of E2F6 by Western blot analysis. E2F6 is detected in nuclear extracts
Protocols
Datasheets and documents
References
This product has been referenced in:
- Pei Y et al. EBV Nuclear Antigen 3C Mediates Regulation of E2F6 to Inhibit E2F1 Transcription and Promote Cell Proliferation. PLoS Pathog 12:e1005844 (2016). Read more (PubMed: 27548379) »
- Hansen HG et al. Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells. Sci Rep 5:18016 (2015). Read more (PubMed: 26657798) »
