Anti-E2F6 antibody [TFE61] (ab11952)
![Western blot - Anti-E2F6 antibody [TFE61] (ab11952)](https://www.abcam.com/ps/products/11/ab11952/Images/ACFAF8.jpg "Western blot - Anti-E2F6 antibody [TFE61] (ab11952)")
Key features and details
- Mouse monoclonal [TFE61] to E2F6
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-E2F6 antibody [TFE61]
See all E2F6 primary antibodies -
Description
Mouse monoclonal [TFE61] to E2F6 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein (Human)
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Positive control
- Hela cells or K-562 nuclear extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
TFE61 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11952 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
| Notes |
|---|
ICC/IF: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F-6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase. -
Tissue specificity
Expressed in all tissues examined. Highest levels in placenta, skeletal muscle, heart, ovary, kidney, small intestine and spleen. -
Sequence similarities
Belongs to the E2F/DP family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1876 Human
- Omim: 602944 Human
- SwissProt: O75461 Human
- Unigene: 603093 Human
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Alternative names
- E2F 6 antibody
- E2F binding site modulating activity protein antibody
- E2F transcription factor 6 antibody
see all
Images
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Western blot - Anti-E2F6 antibody [TFE61] (ab11952)
Detection of E2F6 by Western blot analysis. E2F6 is detected in nuclear extracts derived from K-562 cells using a 1/1000 dilution of ab11952.
Detection of E2F6 by Western blot analysis. E2F6 is detected in nuclear extracts
Datasheets and documents
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Datasheet download
References (5)
ab11952 has been referenced in 5 publications.
- Han N et al. LncRNA MATN1-AS1 prevents glioblastoma cell from proliferation and invasion via RELA regulation and MAPK signaling pathway. Ann Transl Med 7:784 (2019). PubMed: 32042800
- Pei Y et al. EBV Nuclear Antigen 3C Mediates Regulation of E2F6 to Inhibit E2F1 Transcription and Promote Cell Proliferation. PLoS Pathog 12:e1005844 (2016). PubMed: 27548379
- Hansen HG et al. Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells. Sci Rep 5:18016 (2015). PubMed: 26657798
- Furuta M et al. miR-124 and miR-203 are epigenetically silenced tumor-suppressive microRNAs in hepatocellular carcinoma. Carcinogenesis 31:766-76 (2010). WB ; Human . PubMed: 19843643
- Kozaki K et al. Exploration of tumor-suppressive microRNAs silenced by DNA hypermethylation in oral cancer. Cancer Res 68:2094-105 (2008). WB ; Human . PubMed: 18381414
