Product nameAnti-E2F7 antibody
See all E2F7 primary antibodies
DescriptionRabbit polyclonal to E2F7
Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Horse, Cow, Cat, Dog, Pig
- SP2/0 cell lysate and mouse stomach tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab56022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay: 1/62500.
|WB||Use a concentration of 0.5 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
FunctionAlong with E2F8, inhibitor of E2F-dependent transcription that is important for the control of the E2F1-TP53 apoptotic pathway. Directly represses E2F1 transcription (By similarity). Binds DNA independently of DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Appears to regulate a subset of E2F-dependent genes whose products are required for normal cell cycle progession.
Sequence similaritiesBelongs to the E2F/DP family.
DomainBoth DNA-binding domains are required for DNA-binding and are proposed to form an intramolecular structure that is similar to the winged helix structure of the E2F-DP heterodimer.
- Information by UniProt
FormThere are 2 isoforms produced by alternative splicing.
- E2F 7 antibody
- E2F transcription factor 7 antibody
- E2F-7 antibody
Anti-E2F7 antibody (ab56022) at 0.5 µg/ml + SP2/0 cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 99 kDa
Observed band size: 99 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse stomach tissue labelling E2F7 with ab56022 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the fundic gland. Magnification: 400X.
This product has been referenced in:
- Yin W et al. Elevated E2F7 expression predicts poor prognosis in human patients with gliomas. J Clin Neurosci 33:187-193 (2016). Read more (PubMed: 27460513) »
- Mariani SA et al. CDKN2A-independent role of BMI1 in promoting growth and survival of Ph+ acute lymphoblastic leukemia. Leukemia 30:1682-90 (2016). WB . Read more (PubMed: 27125204) »