Anti-E2F8 antibody (ab109596)
Key features and details
- Rabbit polyclonal to E2F8
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-E2F8 antibody
See all E2F8 primary antibodies -
Description
Rabbit polyclonal to E2F8 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Guinea pig, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
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Immunogen
Synthetic peptide, corresponding to a region between residue 817 and 867 of Human E2F8 ( NP_078956.2)
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Positive control
- WB:293T, HeLa or NIH3T3 whole cell lysate IP: Jurkat whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109596 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 94 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| Notes |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 94 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Along with E2F7, inhibitor of E2F-dependent transcription. Binds DNA independently of DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Directly represses E2F1 transcription. Appears to regulate a subset of E2F-dependent genes whose products are required for normal cell cycle progession. -
Sequence similarities
Belongs to the E2F/DP family. -
Domain
Both DNA-binding domains are required for DNA-binding and are proposed to form an intramolecular structure that is similar to the winged helix structure of the E2F-DP heterodimer. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 79733 Human
- Entrez Gene: 108961 Mouse
- Entrez Gene: 308607 Rat
- Omim: 612047 Human
- SwissProt: A0AVK6 Human
- SwissProt: Q58FA4 Mouse
- Unigene: 523526 Human
- Unigene: 240566 Mouse
see all -
Alternative names
- E2F 8 antibody
- E2F family member 82 antibody
- E2F transcription factor 8 antibody
see all
Images
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Immunoprecipitation - Anti-E2F8 antibody (ab109596)
Detection of E2F8 by Western Blot of Immunprecipitate.
ab109596 at 0.1 µg/mL staining E2F8 in Jurkat whole cell lysate immunoprecipitated using ab109596 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 seconds -
Western blot - Anti-E2F8 antibody (ab109596)All lanes : Anti-E2F8 antibody (ab109596) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 5 µg
Lane 4 : HeLa whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 94 kDa
Exposure time: 3 minutes
Protocols
Datasheets and documents
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Datasheet download
References (9)
ab109596 has been referenced in 9 publications.
- Chan AB et al. Cryptochromes modulate E2F family transcription factors. Sci Rep 10:4077 (2020). PubMed: 32139766
- Ji M et al. Small Nuclear Ribonucleoprotein Polypeptide N Accelerates Malignant Progression and Poor Prognosis in Colorectal Cancer Transcriptionally Regulated by E2F8. Front Oncol 10:561287 (2020). PubMed: 33224876
- Zhou Y et al. The regulatory effect of microRNA-21a-3p on the promotion of telocyte angiogenesis mediated by PI3K (p110a)/AKT/mTOR in LPS induced mice ARDS. J Transl Med 17:427 (2019). PubMed: 31878977
- Jin DH et al. Metformin induces cell cycle arrest at the G1 phase through E2F8 suppression in lung cancer cells. Oncotarget 8:101509-101519 (2017). PubMed: 29254182
- Kent LN et al. E2f8 mediates tumor suppression in postnatal liver development. J Clin Invest 126:2955-69 (2016). PubMed: 27454291
- Drelon C et al. EZH2 is overexpressed in adrenocortical carcinoma and is associated with disease progression. Hum Mol Genet 25:2789-2800 (2016). PubMed: 27149985
- de Bruin A et al. Genome-wide analysis reveals NRP1 as a direct HIF1a-E2F7 target in the regulation of motorneuron guidance in vivo. Nucleic Acids Res : (2015). WB ; Human . PubMed: 26681691
- Weijts BG et al. Atypical E2fs control lymphangiogenesis through transcriptional regulation of Ccbe1 and Flt4. PLoS One 8:e73693 (2013). WB, ChIP . PubMed: 24069224
- Weijts BG et al. E2F7 and E2F8 promote angiogenesis through transcriptional activation of VEGFA in cooperation with HIF1. EMBO J 31:3871-84 (2012). PubMed: 22903062
