Overview

  • Product name
    Anti-E2F8 antibody
    See all E2F8 primary antibodies
  • Description
    Rabbit polyclonal to E2F8
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Guinea pig, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between residue 817 and 867 of Human E2F8 ( NP_078956.2)

  • Positive control
    • 293T, HeLa or NIH3T3 whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab109596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 94 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Along with E2F7, inhibitor of E2F-dependent transcription. Binds DNA independently of DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Directly represses E2F1 transcription. Appears to regulate a subset of E2F-dependent genes whose products are required for normal cell cycle progession.
  • Sequence similarities
    Belongs to the E2F/DP family.
  • Domain
    Both DNA-binding domains are required for DNA-binding and are proposed to form an intramolecular structure that is similar to the winged helix structure of the E2F-DP heterodimer.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • E2F 8 antibody
    • E2F family member 82 antibody
    • E2F transcription factor 8 antibody
    • E2F-8 antibody
    • E2f8 antibody
    • E2F8_HUMAN antibody
    • FLJ23311 antibody
    • Transcription factor E2F8 antibody
    see all

Images

  • Western blot - Anti-E2F8 antibody (ab109596)
    Western blot - Anti-E2F8 antibody (ab109596)
    All lanes : Anti-E2F8 antibody (ab109596) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 5 µg
    Lane 4 : HeLa whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 94 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation - Anti-E2F8 antibody (ab109596)
    Immunoprecipitation - Anti-E2F8 antibody (ab109596)
    Detection of E2F8 by Western Blot of Immunprecipitate.
    ab109596 at 1µg/ml staining E2F8 in HeLa whole cell lysate immunoprecipitated using ab109596 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds

References

This product has been referenced in:
  • Jin DH  et al. Metformin induces cell cycle arrest at the G1 phase through E2F8 suppression in lung cancer cells. Oncotarget 8:101509-101519 (2017). Read more (PubMed: 29254182) »
  • de Bruin A  et al. Genome-wide analysis reveals NRP1 as a direct HIF1a-E2F7 target in the regulation of motorneuron guidance in vivo. Nucleic Acids Res : (2015). WB ; Human . Read more (PubMed: 26681691) »

See all 4 Publications for this product

Publishing research using ab109596? Please let us know so that we can cite the reference in this datasheet.

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