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  1. Link

    e2f8-antibody-ab109596.pdf

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Epigenetics and Nuclear Signaling Transcription Other factors
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Anti-E2F8 antibody (ab109596)

  • Datasheet
Submit a review Submit a question References (9)

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Immunoprecipitation - Anti-E2F8 antibody (ab109596)
  • Western blot - Anti-E2F8 antibody (ab109596)

Key features and details

  • Rabbit polyclonal to E2F8
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Recombinant Human E2F8 protein (ab153513)
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Overview

  • Product name

    Anti-E2F8 antibody
    See all E2F8 primary antibodies
  • Description

    Rabbit polyclonal to E2F8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Guinea pig, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between residue 817 and 867 of Human E2F8 ( NP_078956.2)

  • Positive control

    • WB:293T, HeLa or NIH3T3 whole cell lysate IP: Jurkat whole cell lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cancer
    • Cancer Metabolism
    • Transcriptional control
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Nucleotide metabolism
    • Molecular processes
    • Transcriptional control
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • NIH/3T3 whole cell lysate (ab7179)
  • Recombinant Protein

    • Recombinant Human E2F8 protein (ab153513)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/2000 - 1/10000. Predicted molecular weight: 94 kDa.
IP
Use at 2-5 µg/mg of lysate.
Notes
WB
1/2000 - 1/10000. Predicted molecular weight: 94 kDa.
IP
Use at 2-5 µg/mg of lysate.

Target

  • Function

    Along with E2F7, inhibitor of E2F-dependent transcription. Binds DNA independently of DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Directly represses E2F1 transcription. Appears to regulate a subset of E2F-dependent genes whose products are required for normal cell cycle progession.
  • Sequence similarities

    Belongs to the E2F/DP family.
  • Domain

    Both DNA-binding domains are required for DNA-binding and are proposed to form an intramolecular structure that is similar to the winged helix structure of the E2F-DP heterodimer.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession A0AVK6 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 79733 Human
    • Entrez Gene: 108961 Mouse
    • Entrez Gene: 308607 Rat
    • Omim: 612047 Human
    • SwissProt: A0AVK6 Human
    • SwissProt: Q58FA4 Mouse
    • Unigene: 523526 Human
    • Unigene: 240566 Mouse
    • Unigene: 395068 Mouse
    see all
  • Alternative names

    • E2F 8 antibody
    • E2F family member 82 antibody
    • E2F transcription factor 8 antibody
    • E2F-8 antibody
    • E2f8 antibody
    • E2F8_HUMAN antibody
    • FLJ23311 antibody
    • Transcription factor E2F8 antibody
    see all

Images

  • Immunoprecipitation - Anti-E2F8 antibody (ab109596)
    Immunoprecipitation - Anti-E2F8 antibody (ab109596)

    Detection of E2F8 by Western Blot of Immunprecipitate.
    ab109596 at 0.1 µg/mL staining E2F8 in Jurkat whole cell lysate immunoprecipitated using ab109596 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 seconds

  • Western blot - Anti-E2F8 antibody (ab109596)
    Western blot - Anti-E2F8 antibody (ab109596)
    All lanes : Anti-E2F8 antibody (ab109596) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 5 µg
    Lane 4 : HeLa whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 94 kDa


    Exposure time: 3 minutes

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (9)

Publishing research using ab109596? Please let us know so that we can cite the reference in this datasheet.

ab109596 has been referenced in 9 publications.

  • Chan AB  et al. Cryptochromes modulate E2F family transcription factors. Sci Rep 10:4077 (2020). PubMed: 32139766
  • Ji M  et al. Small Nuclear Ribonucleoprotein Polypeptide N Accelerates Malignant Progression and Poor Prognosis in Colorectal Cancer Transcriptionally Regulated by E2F8. Front Oncol 10:561287 (2020). PubMed: 33224876
  • Zhou Y  et al. The regulatory effect of microRNA-21a-3p on the promotion of telocyte angiogenesis mediated by PI3K (p110a)/AKT/mTOR in LPS induced mice ARDS. J Transl Med 17:427 (2019). PubMed: 31878977
  • Jin DH  et al. Metformin induces cell cycle arrest at the G1 phase through E2F8 suppression in lung cancer cells. Oncotarget 8:101509-101519 (2017). PubMed: 29254182
  • Kent LN  et al. E2f8 mediates tumor suppression in postnatal liver development. J Clin Invest 126:2955-69 (2016). PubMed: 27454291
  • Drelon C  et al. EZH2 is overexpressed in adrenocortical carcinoma and is associated with disease progression. Hum Mol Genet 25:2789-2800 (2016). PubMed: 27149985
  • de Bruin A  et al. Genome-wide analysis reveals NRP1 as a direct HIF1a-E2F7 target in the regulation of motorneuron guidance in vivo. Nucleic Acids Res : (2015). WB ; Human . PubMed: 26681691
  • Weijts BG  et al. Atypical E2fs control lymphangiogenesis through transcriptional regulation of Ccbe1 and Flt4. PLoS One 8:e73693 (2013). WB, ChIP . PubMed: 24069224
  • Weijts BG  et al. E2F7 and E2F8 promote angiogenesis through transcriptional activation of VEGFA in cooperation with HIF1. EMBO J 31:3871-84 (2012). PubMed: 22903062

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