Anti-E2F8 antibody (ab126163)
Key features and details
- Rabbit polyclonal to E2F8
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-E2F8 antibody
See all E2F8 primary antibodies -
Description
Rabbit polyclonal to E2F8 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 429-696 of Human E2F8 (UniProt A0AVK6).
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Positive control
- A431 and HepG2 whole cell lysate (ab7900)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126163 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 94 kDa.
|
| Notes |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 94 kDa. |
Target
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Function
Along with E2F7, inhibitor of E2F-dependent transcription. Binds DNA independently of DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Directly represses E2F1 transcription. Appears to regulate a subset of E2F-dependent genes whose products are required for normal cell cycle progession. -
Sequence similarities
Belongs to the E2F/DP family. -
Domain
Both DNA-binding domains are required for DNA-binding and are proposed to form an intramolecular structure that is similar to the winged helix structure of the E2F-DP heterodimer. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 79733 Human
- Omim: 612047 Human
- SwissProt: A0AVK6 Human
- Unigene: 523526 Human
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Alternative names
- E2F 8 antibody
- E2F family member 82 antibody
- E2F transcription factor 8 antibody
see all
Images
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Western blot - Anti-E2F8 antibody (ab126163)All lanes : Anti-E2F8 antibody (ab126163) at 1/500 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 94 kDa
5% SDS PAGE
References (0)
ab126163 has not yet been referenced specifically in any publications.
