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    e2f8-antibody-ab126163.pdf

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Epigenetics and Nuclear Signaling Transcription Other factors
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Anti-E2F8 antibody (ab126163)

  • Datasheet
  • SDS
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Western blot - Anti-E2F8 antibody (ab126163)

    Key features and details

    • Rabbit polyclonal to E2F8
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human E2F8 protein (ab153513)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-E2F8 antibody
      See all E2F8 primary antibodies
    • Description

      Rabbit polyclonal to E2F8
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Recombinant fragment, corresponding to a region within amino acids 429-696 of Human E2F8 (UniProt A0AVK6).

    • Positive control

      • A431 and HepG2 whole cell lysate (ab7900)

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.00
      Preservative: 0.01% Thimerosal (merthiolate)
      Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Other factors
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Transcription Factors
      • Cancer
      • Cancer Metabolism
      • Transcriptional control
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Nucleotide metabolism
      • Molecular processes
      • Transcriptional control
      • Metabolism
      • Types of disease
      • Cancer

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • A-431 whole cell lysate (ab7909)
    • Recombinant Protein

      • Recombinant Human E2F8 protein (ab153513)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab126163 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    Application Abreviews Notes
    WB
    1/500 - 1/3000. Predicted molecular weight: 94 kDa.
    Notes
    WB
    1/500 - 1/3000. Predicted molecular weight: 94 kDa.

    Target

    • Function

      Along with E2F7, inhibitor of E2F-dependent transcription. Binds DNA independently of DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Directly represses E2F1 transcription. Appears to regulate a subset of E2F-dependent genes whose products are required for normal cell cycle progession.
    • Sequence similarities

      Belongs to the E2F/DP family.
    • Domain

      Both DNA-binding domains are required for DNA-binding and are proposed to form an intramolecular structure that is similar to the winged helix structure of the E2F-DP heterodimer.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession A0AVK6 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 79733 Human
      • Omim: 612047 Human
      • SwissProt: A0AVK6 Human
      • Unigene: 523526 Human
      • Alternative names

        • E2F 8 antibody
        • E2F family member 82 antibody
        • E2F transcription factor 8 antibody
        • E2F-8 antibody
        • E2f8 antibody
        • E2F8_HUMAN antibody
        • FLJ23311 antibody
        • Transcription factor E2F8 antibody
        see all

      Images

      • Western blot - Anti-E2F8 antibody (ab126163)
        Western blot - Anti-E2F8 antibody (ab126163)
        All lanes : Anti-E2F8 antibody (ab126163) at 1/500 dilution

        Lane 1 : A431 whole cell lysate
        Lane 2 : HepG2 whole cell lysate


        Lysates/proteins at 30 µg per lane.

        Predicted band size: 94 kDa



        5% SDS PAGE

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (0)

      Publishing research using ab126163? Please let us know so that we can cite the reference in this datasheet.

      ab126163 has not yet been referenced specifically in any publications.

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