- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-E2F8 antibody
See all E2F8 primary antibodies -
DescriptionRabbit polyclonal to E2F8
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 429-696 of Human E2F8 (UniProt A0AVK6).
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Positive control
- A431 and HepG2 whole cell lysates
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78% PBS, 20% Glycerol, 1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab126163 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 94 kDa. |
Target
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FunctionAlong with E2F7, inhibitor of E2F-dependent transcription. Binds DNA independently of DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Directly represses E2F1 transcription. Appears to regulate a subset of E2F-dependent genes whose products are required for normal cell cycle progession.
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Sequence similaritiesBelongs to the E2F/DP family.
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DomainBoth DNA-binding domains are required for DNA-binding and are proposed to form an intramolecular structure that is similar to the winged helix structure of the E2F-DP heterodimer.
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Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 79733 Human
- Omim: 612047 Human
- SwissProt: A0AVK6 Human
- Unigene: 523526 Human
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Alternative names
- E2F 8 antibody
- E2F family member 82 antibody
- E2F transcription factor 8 antibody
see all
Images
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Western blot - Anti-E2F8 antibody (ab126163)All lanes : Anti-E2F8 antibody (ab126163) at 1/500 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 94 kDa
5% SDS PAGE
Protocols
References
ab126163 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"