Anti-E2F8 antibody (ab126163)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-E2F8 antibody
See all E2F8 primary antibodies -
Description
Rabbit polyclonal to E2F8 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 429-696 of Human E2F8 (UniProt A0AVK6).
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Positive control
- A431 and HepG2 whole cell lysate (ab7900)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% CMIT/MIT 3:1
Constituents: PBS, 1% BSA, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab126163 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 94 kDa. |
Target
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Function
Along with E2F7, inhibitor of E2F-dependent transcription. Binds DNA independently of DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Directly represses E2F1 transcription. Appears to regulate a subset of E2F-dependent genes whose products are required for normal cell cycle progession. -
Sequence similarities
Belongs to the E2F/DP family. -
Domain
Both DNA-binding domains are required for DNA-binding and are proposed to form an intramolecular structure that is similar to the winged helix structure of the E2F-DP heterodimer. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 79733 Human
- Omim: 612047 Human
- SwissProt: A0AVK6 Human
- Unigene: 523526 Human
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Alternative names
- E2F 8 antibody
- E2F family member 82 antibody
- E2F transcription factor 8 antibody
see all
Images
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Western blot - Anti-E2F8 antibody (ab126163)All lanes : Anti-E2F8 antibody (ab126163) at 1/500 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 94 kDa
5% SDS PAGE
Protocols
References
ab126163 has not yet been referenced specifically in any publications.
